Genomic variation within the maize stiff-stalk heterotic germplasm pool.


Journal

The plant genome
ISSN: 1940-3372
Titre abrégé: Plant Genome
Pays: United States
ID NLM: 101273919

Informations de publication

Date de publication:
11 2021
Historique:
received: 14 03 2021
accepted: 06 05 2021
pubmed: 19 7 2021
medline: 29 3 2022
entrez: 18 7 2021
Statut: ppublish

Résumé

The stiff-stalk heterotic group in Maize (Zea mays L.) is an important source of inbreds used in U.S. commercial hybrid production. Founder inbreds B14, B37, B73, and, to a lesser extent, B84, are found in the pedigrees of a majority of commercial seed parent inbred lines. We created high-quality genome assemblies of B84 and four expired Plant Variety Protection (ex-PVP) lines LH145 representing B14, NKH8431 of mixed descent, PHB47 representing B37, and PHJ40, which is a Pioneer Hi-Bred International (PHI) early stiff-stalk type. Sequence was generated using long-read sequencing achieving highly contiguous assemblies of 2.13-2.18 Gbp with N50 scaffold lengths >200 Mbp. Inbred-specific gene annotations were generated using a core five-tissue gene expression atlas, whereas transposable element (TE) annotation was conducted using de novo and homology-directed methodologies. Compared with the reference inbred B73, synteny analyses revealed extensive collinearity across the five stiff-stalk genomes, although unique components of the maize pangenome were detected. Comparison of this set of stiff-stalk inbreds with the original Iowa Stiff Stalk Synthetic breeding population revealed that these inbreds represent only a proportion of variation in the original stiff-stalk pool and there are highly conserved haplotypes in released public and ex-Plant Variety Protection inbreds. Despite the reduction in variation from the original stiff-stalk population, substantial genetic and genomic variation was identified supporting the potential for continued breeding success in this pool. The assemblies described here represent stiff-stalk inbreds that have historical and commercial relevance and provide further insight into the emerging maize pangenome.

Identifiants

pubmed: 34275202
doi: 10.1002/tpg2.20114
doi:

Banques de données

Dryad
['10.5061/dryad.wh70rxwmw']

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

e20114

Informations de copyright

© 2021 The Authors. The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America.

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Auteurs

Nolan Bornowski (N)

Dep. of Plant Biology, Michigan State Univ., 612 Wilson Road, East Lansing, MI, 48824, USA.

Kathryn J Michel (KJ)

Dep. of Agronomy, Univ. of Wisconsin - Madison, 1575 Linden Drive, Madison, WI, 53706, USA.

John P Hamilton (JP)

Dep. of Plant Biology, Michigan State Univ., 612 Wilson Road, East Lansing, MI, 48824, USA.

Shujun Ou (S)

Dep. of Ecology, Evolution, and Organismal Biology, Iowa State Univ., 2200 Osborn Drive, Ames, IA, 50011, USA.

Arun S Seetharam (AS)

Dep. of Ecology, Evolution, and Organismal Biology, Iowa State Univ., 2200 Osborn Drive, Ames, IA, 50011, USA.

Jerry Jenkins (J)

HudsonAlpha Institute for Biotechnology, 601 Genome Way Northwest, Huntsville, AL, 35806, USA.

Jane Grimwood (J)

HudsonAlpha Institute for Biotechnology, 601 Genome Way Northwest, Huntsville, AL, 35806, USA.

Chris Plott (C)

HudsonAlpha Institute for Biotechnology, 601 Genome Way Northwest, Huntsville, AL, 35806, USA.

Shengqiang Shu (S)

U.S. Dep. of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Jayson Talag (J)

Arizona Genomics Institute, School of Plant Sciences, Univ. of Arizona, 1657 E Helen Street, Tucson, AZ, 85721, USA.

Megan Kennedy (M)

U.S. Dep. of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Hope Hundley (H)

U.S. Dep. of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Vasanth R Singan (VR)

U.S. Dep. of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Kerrie Barry (K)

U.S. Dep. of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Chris Daum (C)

U.S. Dep. of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Yuko Yoshinaga (Y)

U.S. Dep. of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Jeremy Schmutz (J)

HudsonAlpha Institute for Biotechnology, 601 Genome Way Northwest, Huntsville, AL, 35806, USA.
U.S. Dep. of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Candice N Hirsch (CN)

Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, 1991 Upper Buford Circle, Saint Paul, MN, 55108, USA.

Matthew B Hufford (MB)

Dep. of Ecology, Evolution, and Organismal Biology, Iowa State Univ., 2200 Osborn Drive, Ames, IA, 50011, USA.

Natalia de Leon (N)

Dep. of Agronomy, Univ. of Wisconsin - Madison, 1575 Linden Drive, Madison, WI, 53706, USA.
Dep. of Energy, Great Lakes Bioenergy Research Center, Univ. of Wisconsin - Madison, 1575 Linden Drive, Madison, WI, 53706, USA.

Shawn M Kaeppler (SM)

Dep. of Agronomy, Univ. of Wisconsin - Madison, 1575 Linden Drive, Madison, WI, 53706, USA.
Dep. of Energy, Great Lakes Bioenergy Research Center, Univ. of Wisconsin - Madison, 1575 Linden Drive, Madison, WI, 53706, USA.
Wisconsin Crop Innovation Center, Univ. of Wisconsin - Madison, 8520 University Green, Middleton, WI, 53562, USA.

C Robin Buell (CR)

Dep. of Plant Biology, Michigan State Univ., 612 Wilson Road, East Lansing, MI, 48824, USA.
Dep. of Energy, Great Lakes Bioenergy Research Center, Michigan State Univ., 612 Wilson Road, East Lansing, MI, 48824, USA.

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