Comparative Assessment of Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry (MALDI-TOF-MS) and Conventional Methods in the Identification of Clinically Relevant Yeasts.
candida
cma
identification
maldi-tof-ms
yeast
Journal
Cureus
ISSN: 2168-8184
Titre abrégé: Cureus
Pays: United States
ID NLM: 101596737
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
accepted:
11
06
2021
entrez:
19
7
2021
pubmed:
20
7
2021
medline:
20
7
2021
Statut:
epublish
Résumé
Candida species are generally identified by conventional methods such as germ tube or morphological appearance on cornmeal agar (CMA), biochemical methods using API kits, and molecular biological techniques. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) has revolutionized the identification of fungi reducing the turnaround time of days to minutes. To compare the performance of MALDI-TOF MS and conventional methods in the identification of clinically relevant yeasts. In this study, Candida identifications on CMA are compared with the results obtained on MALDI-TOF MS (Bruker Daltonics, Bremen, Germany). Discrepant results were confirmed by sequencing internal transcribed spacer (ITS) regions of rDNA. A total of 114 clinical Candida species isolated from blood cultures were isolated and identified with conventional methods as well as with the MALDI-TOF-MS system. The agreement between the two test results were analyzed using Inter-rater reliability analysis (Cohen's Kappa) in SPSS Software Version 24 (IBM Corp., Armonk, NY). Overall, there was substantial agreement (Cohen's kappa=0.763) between the two methods. A value between 0.61 and 0.80 is classified under substantial. The most frequently isolated bloodstream Candida species included
Sections du résumé
BACKGROUND
BACKGROUND
Candida species are generally identified by conventional methods such as germ tube or morphological appearance on cornmeal agar (CMA), biochemical methods using API kits, and molecular biological techniques. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) has revolutionized the identification of fungi reducing the turnaround time of days to minutes.
PURPOSE
OBJECTIVE
To compare the performance of MALDI-TOF MS and conventional methods in the identification of clinically relevant yeasts.
MATERIALS AND METHODS
METHODS
In this study, Candida identifications on CMA are compared with the results obtained on MALDI-TOF MS (Bruker Daltonics, Bremen, Germany). Discrepant results were confirmed by sequencing internal transcribed spacer (ITS) regions of rDNA.
RESULTS
RESULTS
A total of 114 clinical Candida species isolated from blood cultures were isolated and identified with conventional methods as well as with the MALDI-TOF-MS system. The agreement between the two test results were analyzed using Inter-rater reliability analysis (Cohen's Kappa) in SPSS Software Version 24 (IBM Corp., Armonk, NY). Overall, there was substantial agreement (Cohen's kappa=0.763) between the two methods. A value between 0.61 and 0.80 is classified under substantial. The most frequently isolated bloodstream Candida species included
Identifiants
pubmed: 34277227
doi: 10.7759/cureus.15607
pmc: PMC8273367
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e15607Informations de copyright
Copyright © 2021, Meena et al.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
J Clin Microbiol. 2009 Sep;47(9):2912-7
pubmed: 19571014
Diagn Microbiol Infect Dis. 2012 May;73(1):65-7
pubmed: 22578939
BMC Infect Dis. 2013 Jul 22;13:337
pubmed: 23875950
Euro Surveill. 2019 Jan;24(4):
pubmed: 30696525
J Clin Microbiol. 2011 Apr;49(4):1614-6
pubmed: 21270234
Clin Microbiol Infect. 2014 Feb;20(2):153-8
pubmed: 23594150
Future Sci OA. 2018 Jun 14;4(6):FSO307
pubmed: 30057784
Med Mycol. 2014 May;52(4):342-9
pubmed: 24782106
BMC Microbiol. 2017 May 25;17(1):128
pubmed: 28545528
Antimicrob Agents Chemother. 2017 Apr 24;61(5):
pubmed: 28264843
Mycopathologia. 2016 Feb;181(1-2):67-73
pubmed: 26400863
Clin Microbiol Infect. 2010 Nov;16(11):1614-9
pubmed: 20636422