A quantitative image-based protocol for morphological characterization of cellular solids in feather shafts.

Animals Chickens Feathers / cytology Image Processing, Computer-Assisted / methods Morphogenesis Paraffin Embedding

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 09 2021
Historique:
entrez: 19 7 2021
pubmed: 20 7 2021
medline: 20 7 2021
Statut: epublish

Résumé

During morphogenesis, cellular sheets undergo dynamic folding to build functional forms. Here, we develop an image-based quantitative morphology field (QMorF) protocol that quantifies the morphological features of cellular structures and associated distributions. Using feather shafts with different rigidities as examples, QMorF performs coarse-graining statistical measurements of the fitted cellular objects over a micro-image stack, revealing underlying mechanical coupling and developmental clues. These images give intuitive representations of mechanical forces and should be useful for analyzing tissue images showing clear cellular features. For complete details on the use and execution of this protocol, please refer to Chang et al. (2019).

Identifiants

DOI: 10.1016/j.xpro.2021.100661 PMID: 34278338 PMC: PMC8271172
pubmed: 34278338
doi: 10.1016/j.xpro.2021.100661
pii: S2666-1667(21)00368-3
pmc: PMC8271172
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100661

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

Applications for patents on our main methodology, referred to as QMorF in the manuscript, are pending in Taiwan and in the United States of America. C.-M.C. is a non-paid science advisor of Integrative Stem Cell Center, CMU/hospital.

Références

Cell. 2019 Nov 27;179(6):1409-1423.e17
pubmed: 31778655
Curr Opin Genet Dev. 2021 Mar 26;69:103-111
pubmed: 33780743

Auteurs

Hao Wu (H)

Integrative Stem Cell Center, China Medical University Hospital, Taichung 40447, Taiwan.
Institute of Physics, Academia Sinica, Taipei 11529, Taiwan.
The iEGG and Animal Biotechnology Research Center, National Chung Hsing University, Taichung 40227, Taiwan.
Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan.

Yu-Kun Chiu (YK)

Institute of Physics, Academia Sinica, Taipei 11529, Taiwan.

Jih-Chiang Tsai (JC)

Institute of Physics, Academia Sinica, Taipei 11529, Taiwan.

Cheng-Ming Chuong (CM)

Department of Pathology, University of Southern California, Los Angeles, CA 90033, USA.

Wen-Tau Juan (WT)

Integrative Stem Cell Center, China Medical University Hospital, Taichung 40447, Taiwan.
Institute of Physics, Academia Sinica, Taipei 11529, Taiwan.
Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 40402, Taiwan.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A quantitative image-based protocol for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Oiseaux › Poulets › A quantitative image-based protocol for...
questionsmedicales.fr › Structures anatomiques de l'animal › Plumes › A quantitative image-based protocol for...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › A quantitative image-based protocol for...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › A quantitative image-based protocol for...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Inclusion de tissu › Inclusion en paraffine › A quantitative image-based protocol for...