Biocompatibility Parameters with Standard and Increased Dose of Citrate in Hemodialysis-A Randomized Trial.

biocompatibility complement system hemodialysis myeloperoxidase platelet factor 4 regional citrate anticoagulation thrombin–antithrombin complex

Journal

Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588

Informations de publication

Date de publication:
04 Jul 2021
Historique:
received: 07 06 2021
revised: 23 06 2021
accepted: 01 07 2021
entrez: 19 7 2021
pubmed: 20 7 2021
medline: 20 7 2021
Statut: epublish

Résumé

The dose of citrate needed in regional citrate anticoagulation (RCA) to achieve optimal biocompatibility is unknown. We performed a randomized trial comparing two doses (ACTRN12613001340729). In 30 patients a single hemodialysis with either standard (2.7 mmol/L) or increased dose of citrate (4 mmol/L) was performed. C5a-desArg, myeloperoxidase (MPO), thrombin-antithrombin complex (TAT), and platelet factor 4 (PF4) were measured and the inner surface of the dialyzer fibers was evaluated with scanning electron microscopy (SEM). A good separation of anticoagulation effect was achieved (post-filter ionized calcium 0.20 vs. 0.31 mmol/L, Given the excellent results in both groups, absence of between group differences and inability of the increased dose of citrate to completely blunt the small residual increase in PF4 and TAT, we conclude that the standard dose of citrate seems sufficient in RCA for chronic hemodialysis.

Sections du résumé

BACKGROUND BACKGROUND
The dose of citrate needed in regional citrate anticoagulation (RCA) to achieve optimal biocompatibility is unknown. We performed a randomized trial comparing two doses (ACTRN12613001340729).
METHODS METHODS
In 30 patients a single hemodialysis with either standard (2.7 mmol/L) or increased dose of citrate (4 mmol/L) was performed. C5a-desArg, myeloperoxidase (MPO), thrombin-antithrombin complex (TAT), and platelet factor 4 (PF4) were measured and the inner surface of the dialyzer fibers was evaluated with scanning electron microscopy (SEM).
RESULTS RESULTS
A good separation of anticoagulation effect was achieved (post-filter ionized calcium 0.20 vs. 0.31 mmol/L,
CONCLUSIONS CONCLUSIONS
Given the excellent results in both groups, absence of between group differences and inability of the increased dose of citrate to completely blunt the small residual increase in PF4 and TAT, we conclude that the standard dose of citrate seems sufficient in RCA for chronic hemodialysis.

Identifiants

pubmed: 34279471
pii: jcm10132987
doi: 10.3390/jcm10132987
pmc: PMC8268353
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Alesa Orsag (A)

Department of Nephrology, University Medical Center Ljubljana, 1000 Ljubljana, Slovenia.
Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.

Mojca Bozic-Mijovski (M)

Department of Vascular Diseases, University Medical Center Ljubljana, 1000 Ljubljana, Slovenia.

Samo Hudoklin (S)

Faculty of Medicine, Institute of Cell Biology, University of Ljubljana, 1000 Ljubljana, Slovenia.

Sasa Simcic (S)

Faculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, 1000 Ljubljana, Slovenia.

Jakob Gubensek (J)

Department of Nephrology, University Medical Center Ljubljana, 1000 Ljubljana, Slovenia.
Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.

Classifications MeSH