Steroid sulfatase in the mouse NIH-3T3 fibroblast cell line: Characterization, and downregulation by glucocorticoids.

Cortisol Estrogens Estrone sulfate Fibroblasts Glucocorticoids Mouse NIH-3T3 cells Regulation Steroid sulfatase Sulfated steroids

Journal

Steroids
ISSN: 1878-5867
Titre abrégé: Steroids
Pays: United States
ID NLM: 0404536

Informations de publication

Date de publication:
10 2021
Historique:
received: 21 12 2020
revised: 03 06 2021
accepted: 06 07 2021
pubmed: 20 7 2021
medline: 25 2 2022
entrez: 19 7 2021
Statut: ppublish

Résumé

Steroid hormones often circulate in the blood as inactive sulfated forms, such as estrone sulfate and dehydroepiandrosterone sulfate. The enzyme steroid sulfatase (STS) converts these steroids into active forms, mainly estrogens, in peripheral tissues. We have previously characterized STS activity in human and mouse breast and bone tissues, and we have shown that STS can provide estrogens to these tissues from circulating sulfated precursors. This study was designed to characterize STS activity in a mouse fibroblast cell line (NIH-3T3). Using a radioactive estrone sulfate (E

Identifiants

pubmed: 34280393
pii: S0039-128X(21)00102-1
doi: 10.1016/j.steroids.2021.108890
pii:
doi:

Substances chimiques

Steryl-Sulfatase EC 3.1.6.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

108890

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Kyle Selcer (K)

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA. Electronic address: selcer@duq.edu.

Barathi Balasubramonian (B)

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA.

Dylan Miller (D)

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA.

Jade Kerr (J)

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA.

Mia DiFrancesco (M)

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA.

Sanjana Ojha (S)

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA.

Rachel Urbano (R)

Department of Biological Sciences, Duquesne University, Pittsburgh, PA, USA.

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Classifications MeSH