Identification of prenylated phenolics in mulberry leaf and their neuroprotective activity.
Antioxidant defense
Ferroptosis
Neuprotective mechanism
Oxidant activity
UPLC-MS/MS
Journal
Phytomedicine : international journal of phytotherapy and phytopharmacology
ISSN: 1618-095X
Titre abrégé: Phytomedicine
Pays: Germany
ID NLM: 9438794
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
16
03
2021
revised:
10
05
2021
accepted:
22
06
2021
pubmed:
21
7
2021
medline:
30
9
2021
entrez:
20
7
2021
Statut:
ppublish
Résumé
Neurodegenerative diseases are becoming increasingly prevalent over the world. Therefore, drug development in this field is urgently required. Neuron impairment leads to the pathogenesis of neurodegenerative diseases, while amelioration of oxidative stress can inhibit the impairment. As a traditional Chinese medicine, mulberry leaf exhibits various pharmacological properties, including neuroprotective activity. But the major components responsible for the neuroprotective activity of mulberry leaf remained unknown. Phytochemicals were potent candidates of neuroprotective drug. Prenylated phenolics are the leading phytochemicals present in mulberry leaf. The aim of this study was to investigate the neuroprotective activities and mechanisms of prenylated phenolics. The chemical structure of isolated compounds were elucidated by MS and NMR. UPLC-MS/MS was used to determine the contents of prenylated phenolics in fresh mulberry leaf. Neurotoxicity was induced by erastin in HT22 cells. CCK-8 assay was performed to assess cell viability. ROS production, GSH level and iron release were monitored by using DCFH-DA, monobromobimane, and FeRhoNox™-1, respectively. qRT-PCR and Western blotting assays were performed to assess gene and protein expression, respectively. Four prenylated phenolics, including isobavachalcone, morachalcone B, moracin N and morachalcone A were isolated and identified from mulberry leaf. Their levels in fresh mulberry leaf were in a decreasing order, moracin N > morachalcone A > morachalcone B > isobavachalcone. Moreover, moracin N showed a good neuroprotective activity with an EC The results confirmed that moracin N was a good ferroptosis inhibitor, which exerted neuroprotective activity through preventing from oxidative stress.
Sections du résumé
BACKGROUND
BACKGROUND
Neurodegenerative diseases are becoming increasingly prevalent over the world. Therefore, drug development in this field is urgently required. Neuron impairment leads to the pathogenesis of neurodegenerative diseases, while amelioration of oxidative stress can inhibit the impairment. As a traditional Chinese medicine, mulberry leaf exhibits various pharmacological properties, including neuroprotective activity. But the major components responsible for the neuroprotective activity of mulberry leaf remained unknown. Phytochemicals were potent candidates of neuroprotective drug. Prenylated phenolics are the leading phytochemicals present in mulberry leaf.
PURPOSE
OBJECTIVE
The aim of this study was to investigate the neuroprotective activities and mechanisms of prenylated phenolics.
METHODS
METHODS
The chemical structure of isolated compounds were elucidated by MS and NMR. UPLC-MS/MS was used to determine the contents of prenylated phenolics in fresh mulberry leaf. Neurotoxicity was induced by erastin in HT22 cells. CCK-8 assay was performed to assess cell viability. ROS production, GSH level and iron release were monitored by using DCFH-DA, monobromobimane, and FeRhoNox™-1, respectively. qRT-PCR and Western blotting assays were performed to assess gene and protein expression, respectively.
RESULTS
RESULTS
Four prenylated phenolics, including isobavachalcone, morachalcone B, moracin N and morachalcone A were isolated and identified from mulberry leaf. Their levels in fresh mulberry leaf were in a decreasing order, moracin N > morachalcone A > morachalcone B > isobavachalcone. Moreover, moracin N showed a good neuroprotective activity with an EC
CONCLUSION
CONCLUSIONS
The results confirmed that moracin N was a good ferroptosis inhibitor, which exerted neuroprotective activity through preventing from oxidative stress.
Identifiants
pubmed: 34281775
pii: S0944-7113(21)00184-7
doi: 10.1016/j.phymed.2021.153641
pii:
doi:
Substances chimiques
Neuroprotective Agents
0
Phenols
0
Phytochemicals
0
Reactive Oxygen Species
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
153641Informations de copyright
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