Effects of Kumaizasa (
Animals
Cytokines
/ genetics
Extracellular Signal-Regulated MAP Kinases
/ immunology
HEK293 Cells
Humans
Immunity, Innate
/ drug effects
JNK Mitogen-Activated Protein Kinases
/ immunology
Lipopolysaccharides
/ pharmacology
Macrophages
/ drug effects
Mice
Plant Extracts
/ pharmacology
Plant Leaves
RAW 264.7 Cells
Sasa
HEK293
Kumaizasa
RAW 264.7
Sasa senanensis
c-Jun N-terminal kinase
extracellular signal-regulated kinase
interleukin-1β
toll-like receptor 2
toll-like receptor 4
tumor necrosis factor-α
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
22
05
2021
revised:
22
06
2021
accepted:
28
06
2021
entrez:
20
7
2021
pubmed:
21
7
2021
medline:
30
7
2021
Statut:
ppublish
Résumé
We investigated the effect of Kumaizasa leaf extract (KLE) on innate immunity using the HEK293 and RAW 264.7 cell lines. KLE, lipopolysaccharides (LPS), or KLE with LPS were added to RAW 264.7 cells. The TNF-α and IL-1β mRNA expression was then quantified. The expression of MAPKs, NFĸB, TNF-α and IL-1β proteins was also quantified. In addition, KLE was added to HEK293 cells and the IL-8 concentration was measured. In RAW 264.7 cells, KLE increased the levels of TNF-α and IL-1β mRNA. By contrast, when KLE and LPS were added to RAW 264.7 cells, the increase in TNF-α and IL-1β mRNA was ameliorated. Similarly, the expression of JNK and ERK proteins was reduced. The addition of KLE to HEK293 cells induced IL-8 production. Based on these results, a KLE-mediated mechanism may regulate immunity by suppressing the expression of JNK and ERK, which are involved in inflammatory signal transduction.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
We investigated the effect of Kumaizasa leaf extract (KLE) on innate immunity using the HEK293 and RAW 264.7 cell lines.
MATERIALS AND METHODS
METHODS
KLE, lipopolysaccharides (LPS), or KLE with LPS were added to RAW 264.7 cells. The TNF-α and IL-1β mRNA expression was then quantified. The expression of MAPKs, NFĸB, TNF-α and IL-1β proteins was also quantified. In addition, KLE was added to HEK293 cells and the IL-8 concentration was measured.
RESULTS
RESULTS
In RAW 264.7 cells, KLE increased the levels of TNF-α and IL-1β mRNA. By contrast, when KLE and LPS were added to RAW 264.7 cells, the increase in TNF-α and IL-1β mRNA was ameliorated. Similarly, the expression of JNK and ERK proteins was reduced. The addition of KLE to HEK293 cells induced IL-8 production.
CONCLUSION
CONCLUSIONS
Based on these results, a KLE-mediated mechanism may regulate immunity by suppressing the expression of JNK and ERK, which are involved in inflammatory signal transduction.
Identifiants
pubmed: 34281880
pii: 41/8/4093
doi: 10.21873/anticanres.15212
doi:
Substances chimiques
Cytokines
0
Lipopolysaccharides
0
Plant Extracts
0
Extracellular Signal-Regulated MAP Kinases
EC 2.7.11.24
JNK Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4093-4100Informations de copyright
Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.