Study of Uptake Mechanisms of Halloysite Nanotubes in Different Cell Lines.
biocompatibility
cellular internalization
endocytic pathway
halloysite nanotubes
hemocompatibility
Journal
International journal of nanomedicine
ISSN: 1178-2013
Titre abrégé: Int J Nanomedicine
Pays: New Zealand
ID NLM: 101263847
Informations de publication
Date de publication:
2021
2021
Historique:
received:
29
01
2021
accepted:
17
05
2021
entrez:
21
7
2021
pubmed:
22
7
2021
medline:
5
10
2021
Statut:
epublish
Résumé
Halloysite nanotubes (HNTs) are a natural aluminosilicate clay with a chemical formula of Al The HNT/CURBO hybrid system, where a fluorescent probe (CURBO) is confined in the HNT lumen, has been used as a model to study the transport pathway mechanisms of HNTs. The cytocompatibility of HNT/CURBO on cell lines model was investigated by MTS assay. In order to identify the internalization pathway involved in the cellular uptake, we performed various endocytosis-inhibiting studies, and we used fluorescence microscopy to verify the nanomaterial internalization by cells. We evaluated the haemolytic effect of HNT/CURBO placed in contact with human red blood cells (HRBCs), by reading the absorbance value of the supernatant at 570 nm. The HNT/CURBO is highly biocompatible and does not have an appreciable haemolytic effect. The results of the inhibition tests have shown that the internalization process of nanotubes occurs in an energy-dependent manner in both the investigated cell lines, although they have different characteristics. In particular, in non-phagocytic cells, clathrin-dependent and independent endocytosis are involved. In phagocytic cells, in addition to phagocytosis and clathrin-dependent endocytosis, microtubules also participate in the halloysite cellular trafficking. Upon internalization by cells, HNT/CURBO is localized in the cytoplasmic area, particularly in the perinuclear region. Understanding the cellular transport pathways of HNTs can help in the rational design of novel drug delivery systems and can be of great value for their applications in biotechnology.
Identifiants
pubmed: 34285481
doi: 10.2147/IJN.S303816
pii: 303816
pmc: PMC8285245
doi:
Substances chimiques
Aluminum Silicates
0
Pharmaceutical Preparations
0
Clay
T1FAD4SS2M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4755-4768Informations de copyright
© 2021 Biddeci et al.
Déclaration de conflit d'intérêts
The authors report no conflicts of interest in this work.
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