Assessment of the Bacterial Pollution and Detection of Antibiotic Resistance Genes in Benin: Case of the Hydrographic Channel Complex Cotonou-Nokoué Lake.


Journal

Journal of environmental and public health
ISSN: 1687-9813
Titre abrégé: J Environ Public Health
Pays: United States
ID NLM: 101516361

Informations de publication

Date de publication:
2021
Historique:
received: 18 12 2020
revised: 09 06 2021
accepted: 28 06 2021
entrez: 21 7 2021
pubmed: 22 7 2021
medline: 4 11 2021
Statut: epublish

Résumé

The study aims to document the level of contamination of the aquatic ecosystem of the Cotonou-Lake Nokoué canal hydrographic complex by multidrug-resistant bacteria and their resistance genes. For this purpose, water samples were taken from several points of the complex and from the sediments at the depth of the lake. Samples of several species of freshly caught fish products from the lake were also collected. Bacteriological analyses were carried out according to the AFNOR standard (NF U: 47-100). The identification of the different bacterial species isolated was then carried out using the API 20E gallery and specific biochemical tests. The antibiogram of the strains was performed according to the recommendations of the EUCAST. Molecular characterization of the identified strains was carried out by searching for resistance and virulence genes. The results obtained revealed the presence of several bacterial species in water samples and in sediment and intestine samples of fishery products with a predominance of Gram-negative bacilli. The resistance profile of Gram-negative bacilli showed a total resistance to metronidazole (100%). 23% of the strains were also resistant to ciprofloxacin, 41% to amoxicillin, and 60% to aztreonam. Of the Gram-positive cocci identified, 66% was resistant to vancomycin, 7.5% to ciprofloxacin, 71% to erythromycin, and 22% to tetracycline. Regarding the genes sought,

Identifiants

pubmed: 34285697
doi: 10.1155/2021/6664816
pmc: PMC8275426
doi:

Substances chimiques

Anti-Bacterial Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

6664816

Informations de copyright

Copyright © 2021 Oloufemi Daniel Ichola et al.

Déclaration de conflit d'intérêts

The authors declare that they have no conflicts of interest.

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Auteurs

Oloufemi Daniel Ichola (OD)

Research and Training Laboratory in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Godomey, Benin.

Victorien Tamegnon Dougnon (VT)

Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Godomey, Benin.

Charles Hornel Koudokpon (CH)

Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Godomey, Benin.

Alidehou Jerrold Agbankpe (AJ)

Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Godomey, Benin.

Esther Deguenon (E)

Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Godomey, Benin.

Aime Cezaire Ayena (AC)

Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Godomey, Benin.

Henri Houenoukpo Soclo (HH)

Research and Training Laboratory in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Godomey, Benin.

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