Seroprevalence of SARS-CoV-2 antibodies among rural healthcare workers.
Adolescent
Adult
Antibodies, Neutralizing
/ blood
Antibodies, Viral
/ blood
COVID-19
/ blood
Female
Fluorescent Antibody Technique
Health Personnel
/ statistics & numerical data
Humans
Male
Middle Aged
Minnesota
/ epidemiology
Neutralization Tests
Rural Health Services
/ statistics & numerical data
SARS-CoV-2
/ immunology
Seroepidemiologic Studies
South Dakota
/ epidemiology
Young Adult
COVID-19
SARS-CoV-2
healthcare worker
rural
seroprevalence
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
received:
04
06
2021
accepted:
17
07
2021
pubmed:
22
7
2021
medline:
28
10
2021
entrez:
21
7
2021
Statut:
ppublish
Résumé
The objective of this longitudinal cohort study was to determine the seroprevalence of antibodies to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in healthcare workers employed at healthcare settings in three rural counties in eastern South Dakota and western Minnesota from May 13, 2020, through December 22, 2020. Three blood draws were performed at five clinical sites and tested for the presence of antibodies against the SARS-CoV-2. Serum samples were tested for the presence of antibodies using a fluorescent microsphere immunoassay (FMIA), neutralization of SARS-CoV-2 spike-pseudotyped particles (SARS-CoV-2pp) assay, and serum virus neutralization (SVN) assay. The seroprevalence was determined to be 1/336 (0.29%) for samples collected from 5/13/20 to 7/13/20, 5/260 (1.92%) for samples collected from 8/13/20 to 9/25/20, and 35/235 (14.89%) for samples collected from 10/16/20 to 12/22/20. Eight of the 35 (22.8%) seropositive individuals identified in the final draw did not report a previous diagnosis with COVID-19. There was a high correlation (>90%) between the FMIA and virus neutralization assays. Each clinical site's seroprevalence was higher than the cumulative incidence for the general public in the respective county as reported by state public health agencies. As of December 2020, there was a high percentage (85%) of seronegative individuals in the study population.
Identifiants
pubmed: 34289148
doi: 10.1002/jmv.27224
pmc: PMC8426917
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6611-6618Subventions
Organisme : BioSNTR
Organisme : South Dakota Governors Office of Economic Development
Organisme : South Dakota Agriculture Experiment Station
Informations de copyright
© 2021 Wiley Periodicals LLC.
Références
Mem Inst Oswaldo Cruz. 2016 Jul 4;111(8):501-4
pubmed: 27384081
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
BMC Vet Res. 2015 Aug 01;11:180
pubmed: 26232106
PLoS One. 2009 Aug 06;4(8):e6529
pubmed: 19657394
Nature. 2020 Aug;584(7821):430-436
pubmed: 32640463
J Med Virol. 2021 Dec;93(12):6611-6618
pubmed: 34289148
Nat Commun. 2021 Apr 9;12(1):2117
pubmed: 33837204
Cell Rep. 2021 Feb 2;34(5):108699
pubmed: 33485405
J Gen Virol. 2016 Dec;97(12):3267-3279
pubmed: 27902357
BMC Vet Res. 2016 Jun 08;12:95
pubmed: 27277214
Aerosol Sci Technol. 2020 Apr 03;0(0):1-4
pubmed: 32308568
Nat Commun. 2021 Feb 10;12(1):905
pubmed: 33568663
JAMA. 2020 Sep 1;324(9):893-895
pubmed: 32780804
JAMA Netw Open. 2020 Dec 1;3(12):e2029058
pubmed: 33301018
Clin Vaccine Immunol. 2012 Feb;19(2):180-9
pubmed: 22155765
N Engl J Med. 2020 Mar 5;382(10):929-936
pubmed: 32004427
Science. 2018 Oct 5;362(6410):75-79
pubmed: 30287659
J King Saud Univ Sci. 2021 May;33(3):101366
pubmed: 33613011
Nat Microbiol. 2020 Apr;5(4):536-544
pubmed: 32123347
Acta Biomed. 2020 Mar 19;91(1):157-160
pubmed: 32191675
Nat Commun. 2020 Mar 27;11(1):1620
pubmed: 32221306
Vaccine. 2010 Jul 19;28(32):5356-64
pubmed: 20488263
MMWR Morb Mortal Wkly Rep. 2020 Nov 20;69(46):1725-1729
pubmed: 33211680