Sarcopenia associates with SNAP-25 SNPs and a miRNAs profile which is modulated by structured rehabilitation treatment.


Journal

Journal of translational medicine
ISSN: 1479-5876
Titre abrégé: J Transl Med
Pays: England
ID NLM: 101190741

Informations de publication

Date de publication:
21 07 2021
Historique:
received: 08 04 2021
accepted: 14 07 2021
entrez: 22 7 2021
pubmed: 23 7 2021
medline: 6 8 2021
Statut: epublish

Résumé

Sarcopenia is a loss of muscle mass and strength causing disability, morbidity, and mortality in older adults, which is characterized by alterations of the neuromuscular junctions (NMJs). SNAP-25 is essential for the maintenance of NMJ integrity, and the expression of this protein was shown to be modulated by the SNAP-25 rs363050 polymorphism and by a number of miRNAs. We analysed these parameters in a cohort of sarcopenic patients undergoing structured rehabilitation. The rs363050 genotype frequency distribution was analyzed in 177 sarcopenic patients and 181 healthy controls (HC). The concentration of seven miRNAs (miR-451a, miR-425-5p, miR155-5p, miR-421-3p, miR-495-3p, miR-744-5p and miR-93-5p), identified by mouse brain miRNome analysis to be differentially expressed in wild type compared to SNAP-25 The SNAP-25 rs363050 AA genotype was significantly more common in sarcopenic patients compared to HC (p These results support the hypothesis of a role for SNAP-25 in sarcopenia and suggest SNAP-25-associated miRNAs as circulatory biomarkers of rehabilitative outcome for sarcopenia.

Sections du résumé

BACKGROUND
Sarcopenia is a loss of muscle mass and strength causing disability, morbidity, and mortality in older adults, which is characterized by alterations of the neuromuscular junctions (NMJs). SNAP-25 is essential for the maintenance of NMJ integrity, and the expression of this protein was shown to be modulated by the SNAP-25 rs363050 polymorphism and by a number of miRNAs.
METHODS
We analysed these parameters in a cohort of sarcopenic patients undergoing structured rehabilitation. The rs363050 genotype frequency distribution was analyzed in 177 sarcopenic patients and 181 healthy controls (HC). The concentration of seven miRNAs (miR-451a, miR-425-5p, miR155-5p, miR-421-3p, miR-495-3p, miR-744-5p and miR-93-5p), identified by mouse brain miRNome analysis to be differentially expressed in wild type compared to SNAP-25
RESULTS
The SNAP-25 rs363050 AA genotype was significantly more common in sarcopenic patients compared to HC (p
CONCLUSION
These results support the hypothesis of a role for SNAP-25 in sarcopenia and suggest SNAP-25-associated miRNAs as circulatory biomarkers of rehabilitative outcome for sarcopenia.

Identifiants

pubmed: 34289870
doi: 10.1186/s12967-021-02989-x
pii: 10.1186/s12967-021-02989-x
pmc: PMC8296538
doi:

Substances chimiques

Biomarkers 0
MIRN495 microRNA, human 0
MicroRNAs 0
SNAP25 protein, human 0
Snap25 protein, mouse 0
Synaptosomal-Associated Protein 25 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

315

Informations de copyright

© 2021. The Author(s).

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Auteurs

Simone Agostini (S)

IRCCS Fondazione Don Carlo Gnocchi ONLUS, P.zza Morandi, 3, 20100, Milan, Italy. sagostini@dongnocchi.it.

Roberta Mancuso (R)

IRCCS Fondazione Don Carlo Gnocchi ONLUS, P.zza Morandi, 3, 20100, Milan, Italy.

Andrea Saul Costa (AS)

IRCCS Fondazione Don Carlo Gnocchi ONLUS, P.zza Morandi, 3, 20100, Milan, Italy.

Franca Rosa Guerini (FR)

IRCCS Fondazione Don Carlo Gnocchi ONLUS, P.zza Morandi, 3, 20100, Milan, Italy.

Fabio Trecate (F)

IRCCS Fondazione Don Carlo Gnocchi ONLUS, P.zza Morandi, 3, 20100, Milan, Italy.

Rossella Miglioli (R)

IRCCS Fondazione Don Carlo Gnocchi ONLUS, P.zza Morandi, 3, 20100, Milan, Italy.

Elisabetta Menna (E)

CNR-Institute of Neuroscience, Milan, Italy.
Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, Italy.

Beatrice Arosio (B)

Geriatric Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy.

Mario Clerici (M)

IRCCS Fondazione Don Carlo Gnocchi ONLUS, P.zza Morandi, 3, 20100, Milan, Italy.
Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy.

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Classifications MeSH