Role of Plasmodium berghei ookinete surface and oocyst capsule protein, a novel oocyst capsule-associated protein, in ookinete motility.
Knock out parasite
Oocyst
Ookinete
Plasmodium berghei
Transmission block
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
21 Jul 2021
21 Jul 2021
Historique:
received:
13
03
2021
accepted:
30
06
2021
entrez:
22
7
2021
pubmed:
23
7
2021
medline:
20
11
2021
Statut:
epublish
Résumé
Plasmodium sp., which causes malaria, must first develop in mosquitoes before being transmitted. Upon ingesting infected blood, gametes form in the mosquito lumen, followed by fertilization and differentiation of the resulting zygotes into motile ookinetes. Within 24 h of blood ingestion, these ookinetes traverse mosquito epithelial cells and lodge below the midgut basal lamina, where they differentiate into sessile oocysts that are protected by a capsule. We identified an ookinete surface and oocyst capsule protein (OSCP) that is involved in ookinete motility as well as oocyst capsule formation. We found that knockout of OSCP in parasite decreases ookinete gliding motility and gradually reduces the number of oocysts. On day 15 after blood ingestion, the oocyst wall was significantly thinner. Moreover, adding anti-OSCP antibodies decreased the gliding speed of wild-type ookinetes in vitro. Adding anti-OSCP antibodies to an infected blood meal also resulted in decreased oocyst formation. These findings may be useful for the development of a transmission-blocking tool for malaria.
Sections du résumé
BACKGROUND
BACKGROUND
Plasmodium sp., which causes malaria, must first develop in mosquitoes before being transmitted. Upon ingesting infected blood, gametes form in the mosquito lumen, followed by fertilization and differentiation of the resulting zygotes into motile ookinetes. Within 24 h of blood ingestion, these ookinetes traverse mosquito epithelial cells and lodge below the midgut basal lamina, where they differentiate into sessile oocysts that are protected by a capsule.
METHODS
METHODS
We identified an ookinete surface and oocyst capsule protein (OSCP) that is involved in ookinete motility as well as oocyst capsule formation.
RESULTS
RESULTS
We found that knockout of OSCP in parasite decreases ookinete gliding motility and gradually reduces the number of oocysts. On day 15 after blood ingestion, the oocyst wall was significantly thinner. Moreover, adding anti-OSCP antibodies decreased the gliding speed of wild-type ookinetes in vitro. Adding anti-OSCP antibodies to an infected blood meal also resulted in decreased oocyst formation.
CONCLUSION
CONCLUSIONS
These findings may be useful for the development of a transmission-blocking tool for malaria.
Identifiants
pubmed: 34289894
doi: 10.1186/s13071-021-04868-2
pii: 10.1186/s13071-021-04868-2
pmc: PMC8296654
doi:
Substances chimiques
Antibodies, Protozoan
0
Protozoan Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
373Subventions
Organisme : JSPS KAKENHI
ID : 17H04073
Organisme : JSPS KAKENHI
ID : 16H05026
Organisme : JSPS KAKENHI
ID : 19K22355
Informations de copyright
© 2021. The Author(s).
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