Single-Molecule Techniques to Study Chromatin.

DNA fiber analysis chromatin accessibility chromatin replication/transcription electron microscopy histone modifications magnetic/optical tweezers single-molecule techniques third-generation sequencing

Journal

Frontiers in cell and developmental biology

ISSN: 2296-634X

Titre abrégé: Front Cell Dev Biol

Pays: Switzerland

ID NLM: 101630250

Informations de publication

Date de publication:
2021

Historique:

received: 24 04 2021

accepted: 26 05 2021

entrez: 22 7 2021

pubmed: 23 7 2021

medline: 23 7 2021

Statut: epublish

Résumé

Besides the basic organization in nucleosome core particles (NCPs), eukaryotic chromatin is further packed through interactions with numerous protein complexes including transcription factors, chromatin remodeling and modifying enzymes. This nucleoprotein complex provides the template for many important biological processes, such as DNA replication, transcription, and DNA repair. Thus, to understand the molecular basis of these DNA transactions, it is critical to define individual changes of the chromatin structure at precise genomic regions where these machineries assemble and drive biological reactions. Single-molecule approaches provide the only possible solution to overcome the heterogenous nature of chromatin and monitor the behavior of individual chromatin transactions in real-time. In this review, we will give an overview of currently available single-molecule methods to obtain mechanistic insights into nucleosome positioning, histone modifications and DNA replication and transcription analysis-previously unattainable with population-based assays.

Identifiants

DOI: 10.3389/fcell.2021.699771 PMID: 34291054 PMC: PMC8287188

pubmed: 34291054

doi: 10.3389/fcell.2021.699771

pmc: PMC8287188

doi:

Types de publication

Journal Article Review

Langues

eng

Pagination

699771

Informations de copyright

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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