Methylation status of nc886 epiallele reflects periconceptional conditions and is associated with glucose metabolism through nc886 RNAs.
Genomic imprinting
Population studies
miR-886
nc886
vtRNA2-1
Journal
Clinical epigenetics
ISSN: 1868-7083
Titre abrégé: Clin Epigenetics
Pays: Germany
ID NLM: 101516977
Informations de publication
Date de publication:
22 07 2021
22 07 2021
Historique:
received:
07
04
2021
accepted:
13
07
2021
entrez:
23
7
2021
pubmed:
24
7
2021
medline:
1
2
2022
Statut:
epublish
Résumé
Non-coding RNA 886 (nc886) is coded from a maternally inherited metastable epiallele. We set out to investigate the determinants and dynamics of the methylation pattern at the nc886 epiallele and how this methylation status associates with nc886 RNA expression. Furthermore, we investigated the associations between the nc886 methylation status or the levels of nc886 RNAs and metabolic traits in the YFS and KORA cohorts. The association between nc886 epiallele methylation and RNA expression was also validated in induced pluripotent stem cell (iPSC) lines. We confirm that the methylation status of the nc886 epiallele is mostly binomial, with individuals displaying either a non- or hemi-methylated status, but we also describe intermediately and close to fully methylated individuals. We show that an individual's methylation status is associated with the mother's age and socioeconomic status, but not with the individual's own genetics. Once established, the methylation status of the nc886 epiallele remains stable for at least 25 years. This methylation status is strongly associated with the levels of nc886 non-coding RNAs in serum, blood, and iPSC lines. In addition, nc886 methylation status associates with glucose and insulin levels during adolescence but not with the indicators of glucose metabolism or the incidence of type 2 diabetes in adulthood. However, the nc886-3p RNA levels also associate with glucose metabolism in adulthood. These results indicate that nc886 metastable epiallele methylation is tuned by the periconceptional conditions and it associates with glucose metabolism through the expression of the ncRNAs coded in the epiallele region.
Sections du résumé
BACKGROUND
Non-coding RNA 886 (nc886) is coded from a maternally inherited metastable epiallele. We set out to investigate the determinants and dynamics of the methylation pattern at the nc886 epiallele and how this methylation status associates with nc886 RNA expression. Furthermore, we investigated the associations between the nc886 methylation status or the levels of nc886 RNAs and metabolic traits in the YFS and KORA cohorts. The association between nc886 epiallele methylation and RNA expression was also validated in induced pluripotent stem cell (iPSC) lines.
RESULTS
We confirm that the methylation status of the nc886 epiallele is mostly binomial, with individuals displaying either a non- or hemi-methylated status, but we also describe intermediately and close to fully methylated individuals. We show that an individual's methylation status is associated with the mother's age and socioeconomic status, but not with the individual's own genetics. Once established, the methylation status of the nc886 epiallele remains stable for at least 25 years. This methylation status is strongly associated with the levels of nc886 non-coding RNAs in serum, blood, and iPSC lines. In addition, nc886 methylation status associates with glucose and insulin levels during adolescence but not with the indicators of glucose metabolism or the incidence of type 2 diabetes in adulthood. However, the nc886-3p RNA levels also associate with glucose metabolism in adulthood.
CONCLUSIONS
These results indicate that nc886 metastable epiallele methylation is tuned by the periconceptional conditions and it associates with glucose metabolism through the expression of the ncRNAs coded in the epiallele region.
Identifiants
pubmed: 34294131
doi: 10.1186/s13148-021-01132-3
pii: 10.1186/s13148-021-01132-3
pmc: PMC8296652
doi:
Substances chimiques
RNA, Untranslated
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
143Subventions
Organisme : Horizon 2020 (Taxinomisis)
ID : 755320
Organisme : German Research Foundation
ID : WA 4081/1-1
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S020845/1
Pays : United Kingdom
Organisme : Academy of Finland
ID : 134309, 126925, 121584, 124282, 129378, 117787, 41071
Organisme : Academy of Finland
ID : 286284 and 322098
Organisme : Joint Programming Initiative A healthy diet for a healthy life (DIMENSION)
ID : 01EA1902A
Organisme : Horizon 2020 (To_Aition)
ID : 848146
Organisme : Tampere University Hospital Medical Funds
ID : 9X047, 9S054, and 9AB059
Organisme : European Research Council (MULTIEPIGEN)
ID : 742927
Organisme : academy of finland
ID : 285902, 330809 and 338395
Organisme : Tampere University Hospital Medical Funds
ID : X51001
Informations de copyright
© 2021. The Author(s).
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