De novo and cell line models of human mammary cell transformation reveal an essential role for Yb-1 in multiple stages of human breast cancer.
Journal
Cell death and differentiation
ISSN: 1476-5403
Titre abrégé: Cell Death Differ
Pays: England
ID NLM: 9437445
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
26
01
2021
accepted:
07
07
2021
revised:
03
07
2021
pubmed:
24
7
2021
medline:
5
4
2022
entrez:
23
7
2021
Statut:
ppublish
Résumé
Breast cancer heterogeneity has made it challenging to identify mechanisms critical to the initial stages of their genesis in vivo. Here, we sought to interrogate the role of YB-1 in newly arising human breast cancers as well as in established cell lines. In a first series of experiments, we found that short-hairpin RNA-mediated knockdown of YB-1 in MDA-MB-231 cells blocked both their local tumour-forming and lung-colonising activity in immunodeficient mice. Conversely, upregulated expression of YB-1 enhanced the poor in vivo tumorigenicity of T47D cells. We then found that YB-1 knockdown also inhibits the initial generation in mice of invasive ductal carcinomas and ductal carcinomas in situ from freshly isolated human mammary cells transduced, respectively, with KRAS
Identifiants
pubmed: 34294889
doi: 10.1038/s41418-021-00836-6
pii: 10.1038/s41418-021-00836-6
pmc: PMC8738742
doi:
Substances chimiques
Poly-ADP-Ribose Binding Proteins
0
RNA Recognition Motif Proteins
0
Transcription Factors
0
Y-Box-Binding Protein 1
0
YB-1 protein, mouse
0
YBX1 protein, human
0
DNA Helicases
EC 3.6.4.-
G3BP1 protein, human
EC 3.6.4.12
RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
54-64Subventions
Organisme : Michael Smith Foundation for Health Research (MSFHR)
ID : 17159
Organisme : Gouvernement du Canada | Instituts de Recherche en Santé du Canada | CIHR Skin Research Training Centre (Skin Research Training Centre)
ID : CRP-154482
Organisme : CIHR
ID : 143280
Pays : Canada
Informations de copyright
© 2021. The Author(s).
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