Updates on Clinical and Genetic Heterogeneity of

MCPH5 Pakistani population founder effect primary microcephaly whole exome sequencing

Journal

Frontiers in pediatrics
ISSN: 2296-2360
Titre abrégé: Front Pediatr
Pays: Switzerland
ID NLM: 101615492

Informations de publication

Date de publication:
2021
Historique:
received: 14 04 2021
accepted: 27 05 2021
entrez: 23 7 2021
pubmed: 24 7 2021
medline: 24 7 2021
Statut: epublish

Résumé

Microcephaly (MCPH) is a genetically heterogeneous disorder characterized by non-progressive intellectual disability, small head circumference, and small brain size compared with the age- and sex-matched population. MCPH manifests as an isolated condition or part of another clinical syndrome; so far, 25 genes have been linked with MCPH. Many of these genes are reported in Pakistani population, but due to a high rate of consanguinity, a significant proportion of MCPH cohort is yet to be explored. MCPH5 is the most frequently reported type, accounting for up to 68.75% alone in a genetically constrained population like Pakistan. In the current study, whole exome sequencing (WES) was performed on probands from 10 families sampled from South Waziristan and two families from rural areas of the Pakistani Punjab. Candidate variants were validated through Sanger sequencing in all available family members. Variant filtering and

Identifiants

pubmed: 34295862
doi: 10.3389/fped.2021.695133
pmc: PMC8290066
doi:

Types de publication

Journal Article

Langues

eng

Pagination

695133

Informations de copyright

Copyright © 2021 Khan, Hussain, Zheng, Khan, Masoud, Gu, Qiu, Malik, Qasim, Tariq and Chang.

Déclaration de conflit d'intérêts

CZ and QG were employed by the company Shenzhen Real Omics Biotech Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Niaz Muhammad Khan (NM)

Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad, Pakistan.

Basharat Hussain (B)

Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
University of Chinese Academy of Sciences, Beijing, China.

Chenqing Zheng (C)

Shenzhen Real Omics Biotech Co., Ltd., Shenzhen, China.

Ayaz Khan (A)

National Institute for Biotechnology and Genetic Engineering (NIBGE-C), Faisalabad, Pakistan; Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan.

Muhammad Shareef Masoud (MS)

Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad, Pakistan.

Qingquan Gu (Q)

Shenzhen Real Omics Biotech Co., Ltd., Shenzhen, China.

Linhui Qiu (L)

Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Naveed Altaf Malik (NA)

National Institute for Biotechnology and Genetic Engineering (NIBGE-C), Faisalabad, Pakistan; Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan.

Muhammad Qasim (M)

Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad, Pakistan.

Muhammad Tariq (M)

National Institute for Biotechnology and Genetic Engineering (NIBGE-C), Faisalabad, Pakistan; Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan.

Junlei Chang (J)

Shenzhen Key Laboratory of Biomimetic Materials and Cellular Immunomodulation, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Classifications MeSH