Development of rELISA using novel markers for the diagnosis of paratuberculosis.
Animals
Antibodies, Bacterial
/ blood
Antigens, Bacterial
/ genetics
Biomarkers
/ blood
Buffaloes
Cattle
Early Diagnosis
Enzyme-Linked Immunosorbent Assay
/ veterinary
Goats
Immunodominant Epitopes
Mycobacterium avium subsp. paratuberculosis
/ immunology
Paratuberculosis
/ blood
Predictive Value of Tests
Recombinant Proteins
/ immunology
Reproducibility of Results
Serologic Tests
/ veterinary
Sheep, Domestic
ELISA
Paratuberculosis
handheld ELISA reader
herd screening assay
recombinant proteins
secretory antigens
Journal
Journal of immunological methods
ISSN: 1872-7905
Titre abrégé: J Immunol Methods
Pays: Netherlands
ID NLM: 1305440
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
12
12
2020
revised:
15
07
2021
accepted:
16
07
2021
pubmed:
24
7
2021
medline:
9
11
2021
entrez:
23
7
2021
Statut:
ppublish
Résumé
Paratuberculosis is one of the complex livestock infections whose control has largely been hampered due to the lack of efficacious diagnostics. Present study optimized plate ELISA assay for the diagnosis and screening of paratuberculosis using recombinant secretory proteins. Five secretory antigens (2677c, 3547c, 4308c, 1693c, and 2168c) were produced in the recombinant system using the E. coli host and used for the optimization of the assay. These proteins were selected because of their prior proven specificity and antigenicity as humoral immunity markers. The assay was first optimized using traditional ELISA reader and then the performance was evaluated using a handheld ELISA reader. Findings were identical in both traditional ELISA reader as well as handheld ELISA reader. Optimized ELISA was found reproducible using different batches of the recombinant antigens as well as in terms of the inter and intra assay %CV values. The present ELISA has a sensitivity and specificity of 91.6% and 100%, respectively. Also, rELISA revealed AUC
Identifiants
pubmed: 34298067
pii: S0022-1759(21)00150-2
doi: 10.1016/j.jim.2021.113105
pii:
doi:
Substances chimiques
Antibodies, Bacterial
0
Antigens, Bacterial
0
Biomarkers
0
Immunodominant Epitopes
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113105Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.