Extension of Collagen Deposition in COVID-19 Post Mortem Lung Samples and Computed Tomography Analysis Findings.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
13 Jul 2021
Historique:
received: 03 06 2021
revised: 30 06 2021
accepted: 09 07 2021
entrez: 24 7 2021
pubmed: 25 7 2021
medline: 31 7 2021
Statut: epublish

Résumé

Lung fibrosis has specific computed tomography (CT) findings and represents a common finding in advanced COVID-19 pneumonia whose reversibility has been poorly investigated. The aim of this study was to quantify the extension of collagen deposition and aeration in postmortem cryobiopsies of critically ill COVID-19 patients and to describe the correlations with qualitative and quantitative analyses of lung CT. Postmortem transbronchial cryobiopsy samples were obtained, formalin fixed, paraffin embedded and stained with Sirius red to quantify collagen deposition, defining fibrotic samples as those with collagen deposition above 10%. Lung CT images were analyzed qualitatively with a radiographic score and quantitatively with computer-based analysis at the lobe level. Thirty samples from 10 patients with COVID-19 pneumonia deceased during invasive mechanical ventilation were included in this study. The median [interquartile range] percent collagen extension was 6.8% (4.6-16.2%). In fibrotic compared to nonfibrotic samples, the qualitative score was higher (260 (250-290) vs. 190 (120-270),

Identifiants

pubmed: 34299124
pii: ijms22147498
doi: 10.3390/ijms22147498
pmc: PMC8305333
pii:
doi:

Substances chimiques

Collagen 9007-34-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Lorenzo Ball (L)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
Anesthesia and Intensive Care, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Emanuela Barisione (E)

Interventional Pulmonology Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Luca Mastracci (L)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
Anatomic Pathology Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Michela Campora (M)

Surgical Pathology Unit, Santa Chiara Hospital, Provincial Agency for Health Services, 38122 Trento, Italy.

Delfina Costa (D)

Molecular Oncology and Angiogenesis Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Chiara Robba (C)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
Anesthesia and Intensive Care, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Denise Battaglini (D)

Anesthesia and Intensive Care, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Marco Micali (M)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.

Federico Costantino (F)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.

Giuseppe Cittadini (G)

Radiology Department, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Nicolò Patroniti (N)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
Anesthesia and Intensive Care, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Paolo Pelosi (P)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
Anesthesia and Intensive Care, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Roberto Fiocca (R)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
Anatomic Pathology Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

Federica Grillo (F)

Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, 16126 Genoa, Italy.
Anatomic Pathology Unit, San Martino Policlinico Hospital, IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy.

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Classifications MeSH