Optimal Treatment of 6-Dimethylaminopurine Enhances the In Vivo Development of Canine Embryos by Rapid Initiation of DNA Synthesis.
Adenine
/ analogs & derivatives
Animals
Cloning, Organism
/ methods
DNA
/ drug effects
DNA Replication
/ drug effects
Dogs
Embryo Transfer
/ methods
Embryo, Mammalian
/ drug effects
Embryonic Development
/ drug effects
Female
Nuclear Transfer Techniques
Oocytes
/ drug effects
Parthenogenesis
/ drug effects
Pregnancy
BrdU incorporation
DMAP
SCNT
dog
parthenogenesis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
20 Jul 2021
20 Jul 2021
Historique:
received:
29
06
2021
revised:
16
07
2021
accepted:
17
07
2021
entrez:
24
7
2021
pubmed:
25
7
2021
medline:
5
8
2021
Statut:
epublish
Résumé
Artificial activation of oocytes is an important step for successful parthenogenesis and somatic cell nuclear transfer (SCNT). Here, we investigated the initiation of DNA synthesis and in vivo development of canine PA embryos and cloned embryos produced by treatment with 1.9 mM 6-dimethylaminopurine (6-DMAP) for different lengths of time. For experiments, oocytes for parthenogenesis and SCNT oocytes were cultured for 4 min in 10 μM calcium ionophore, and then divided into 2 groups: (1) culture for 2 h in 6-DMAP (DMAP-2h group); (2) culture for 4 h in DMAP (DMAP-4h group). DNA synthesis was clearly detected in all parthenogenetic (PA) embryos and cloned embryos incorporated BrdU 4 h after activation in DMAP-2h and DMAP-4h groups. In vivo development of canine parthenogenetic fetuses was observed after embryo transfer and the implantation rates of PA embryos in DMAP-2h were 34%, which was significantly higher than those in DMAP-4h (6.5%,
Identifiants
pubmed: 34299380
pii: ijms22147757
doi: 10.3390/ijms22147757
pmc: PMC8303139
pii:
doi:
Substances chimiques
N(6),N(6)-dimethyladenine
649SA4S2CV
DNA
9007-49-2
Adenine
JAC85A2161
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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