Biotyping and enterotoxigenicity of coagulase-positive and coagulase-negative staphylococci isolated from different raw meat.
Coagulase
Enterotoxin
Meat
PCR
Staphylococcus
Journal
Iranian journal of veterinary research
ISSN: 1728-1997
Titre abrégé: Iran J Vet Res
Pays: Iran
ID NLM: 101660030
Informations de publication
Date de publication:
2021
2021
Historique:
received:
13
04
2020
revised:
28
10
2020
accepted:
25
01
2021
entrez:
26
7
2021
pubmed:
27
7
2021
medline:
27
7
2021
Statut:
ppublish
Résumé
Staphylococcal enterotoxins (SEs) are produced in foods under favorable conditions and considered a potential biological threat. The study was performed to detect enterotoxigenic genes of A and B in coagulase-positive (CPS) and coagulase-negative staphylococci (CNS) to evaluate biotypes and antibiotic resistance of isolated A total of 160 meat swab samples were collected from lamb, water buffalo, cattle, and chicken carcasses. Presumptive colonies on Baird Parker agar were subjected to biochemical identification, including Gram staining, catalase, oxidase, and coagulase activity. Relevant colonies separately were subjected to the polymerase chain reaction (PCR) assay for identification of Totally, 150 The meats provided from this area were contaminated with enterotoxigenic and antibiotic-resistance staphylococci, which can threaten the health of the consumers. The study showed that not only CPS contain enterotoxin A gene, but also CNS isolates possess this gene, especially in buffalo meat, and thus they are recognized as potential hazards in different meats.
Sections du résumé
BACKGROUND
BACKGROUND
Staphylococcal enterotoxins (SEs) are produced in foods under favorable conditions and considered a potential biological threat.
AIMS
OBJECTIVE
The study was performed to detect enterotoxigenic genes of A and B in coagulase-positive (CPS) and coagulase-negative staphylococci (CNS) to evaluate biotypes and antibiotic resistance of isolated
METHODS
METHODS
A total of 160 meat swab samples were collected from lamb, water buffalo, cattle, and chicken carcasses. Presumptive colonies on Baird Parker agar were subjected to biochemical identification, including Gram staining, catalase, oxidase, and coagulase activity. Relevant colonies separately were subjected to the polymerase chain reaction (PCR) assay for identification of
RESULTS
RESULTS
Totally, 150
CONCLUSION
CONCLUSIONS
The meats provided from this area were contaminated with enterotoxigenic and antibiotic-resistance staphylococci, which can threaten the health of the consumers. The study showed that not only CPS contain enterotoxin A gene, but also CNS isolates possess this gene, especially in buffalo meat, and thus they are recognized as potential hazards in different meats.
Identifiants
pubmed: 34306108
doi: 10.22099/ijvr.2021.36953.5392
pmc: PMC8294818
doi:
Types de publication
Journal Article
Langues
eng
Pagination
113-119Déclaration de conflit d'intérêts
The authors declare that they have no conflict of interest.
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