miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1.
NCK adaptor protein 1
microRNA-374a-5p
non-small cell lung cancer
tumor suppressor
Journal
Experimental and therapeutic medicine
ISSN: 1792-1015
Titre abrégé: Exp Ther Med
Pays: Greece
ID NLM: 101531947
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
02
08
2019
accepted:
16
04
2021
entrez:
26
7
2021
pubmed:
27
7
2021
medline:
27
7
2021
Statut:
ppublish
Résumé
Emerging studies have indicated that microRNAs (miRNAs/miRs) are involved in regulating non-small cell lung cancer (NSCLC)-associated processes. The present study aimed to evaluate the biological roles of miR-374a-5p in NSCLC. Using reverse transcription-quantitative PCR, the expression levels of miR-374a-5p were determined in NSCLC cells and a normal cell line. Functional experiments were performed to investigate the functions of miR-374a-5p in NSCLC. A luciferase activity reporter assay and rescue experiments were performed to validate NCK adaptor protein 1 (NCK1) as a functional target of miR-374a-5p. It was demonstrated that miR-374a-5p levels were decreased in NSCLC cell lines compared with those in a normal cell line. Furthermore, overexpression of miR-374a-5p inhibited NSCLC cell proliferation and migration
Identifiants
pubmed: 34306207
doi: 10.3892/etm.2021.10375
pii: ETM-0-0-10375
pmc: PMC8281440
doi:
Types de publication
Journal Article
Langues
eng
Pagination
943Informations de copyright
Copyright: © Guo et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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