Glutathione reductase: A cytoplasmic antioxidant enzyme and a potential target for phenothiazinium dyes in Neospora caninum.
Glutathione reductase
Neospora caninum
Phenothiazinium dyes
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
30 Sep 2021
30 Sep 2021
Historique:
received:
04
12
2020
revised:
21
05
2021
accepted:
15
07
2021
pubmed:
27
7
2021
medline:
4
1
2022
entrez:
26
7
2021
Statut:
ppublish
Résumé
Neospora caninum causes heavy losses related to abortions in bovine cattle. This parasite developed a complex defense redox system, composed of enzymes as glutathione reductase (GR). Methylene blue (MB) impairs the activity of recombinant form of Plasmodium GR and inhibits the parasite proliferation in vivo and in vitro. Likewise, MB and its derivatives inhibits Neospora caninum proliferation, however, whether the MB mechanism of action is correlated to GR function remains unclear. Therefore, here, N. caninum GR (NcGR) was characterized and its potential inhibitors were determined. NcGR was found in the tachyzoite cytosol and has a similar structure and sequence compared to its homologs. We verified the in vitro activity of rNcGR (875 nM) following NADPH absorbance at 340 nM (100 mM KH
Identifiants
pubmed: 34310993
pii: S0141-8130(21)01559-2
doi: 10.1016/j.ijbiomac.2021.07.108
pii:
doi:
Substances chimiques
Coccidiostats
0
Enzyme Inhibitors
0
Tolonium Chloride
15XUH0X66N
new methylene blue
1934-16-3
Glutathione Reductase
EC 1.8.1.7
Methylene Blue
T42P99266K
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
964-975Informations de copyright
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