Development of an enzymatic screening method for d-aspartate-producing lactic acid bacteria.
Enzymatic screening
Lactic acid bacteria
d-aspartate
d-aspartate oxidase
Journal
Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
01
12
2020
revised:
25
05
2021
accepted:
28
05
2021
entrez:
27
7
2021
pubmed:
28
7
2021
medline:
19
8
2021
Statut:
ppublish
Résumé
d-Aspartate (d-Asp) is an important intermediate for synthetic penicillin and an endogenous amino acid that plays important roles in the endocrine and nervous systems in animals including humans. Lactic acid bacteria (LABs) have been used as probiotics in humans, and some LAB species produce d-Asp as a component of cell wall peptidoglycan. LAB strains with greater d-Asp production would therefore be valuable for industrial d-Asp production. In this study, we developed an enzymatic screening method for d-Asp-producing LABs and isolated a strain with high d-Asp production. The d-Asp concentration in the culture medium was colorimetrically estimated up to 4 mM using d-aspartate oxidase (ChDDO) from the yeast Cryptococcus humicola strain UJ1 coupled with horseradish peroxidase, although a more accurate determination required correction because of interference by the medium component Mn
Identifiants
pubmed: 34311880
pii: S0141-0229(21)00093-4
doi: 10.1016/j.enzmictec.2021.109835
pii:
doi:
Substances chimiques
Amino Acids
0
Aspartic Acid
30KYC7MIAI
D-Aspartic Acid
4SR0Q8YD1X
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109835Informations de copyright
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