Infection dynamics of Cryptosporidium bovis and Cryptosporidium ryanae in a Swedish dairy herd.

In order to investigate the infection dynamics of Cryptosporidium bovis and Cryptosporidium ryanae, a Swedish dairy farm known to be free of C. parvum was recruited for a one-year study. Fecal samples were collected every four weeks (a total of 13 occasions) from 20 randomly selected calves younger than 65 days. A total of 238 feces samples were collected directly from the rectum and then examined with immunofluorescence microscopy; those positive for Cryptosporidium spp. oocysts were further processed by molecular species determination. Oocysts were found in 92 samples, and in 72 of these the species was successfully determined: 87.5% C. bovis, 9.7% C. ryanae and 2.8% a mix of both species. No C. parvum was found. The prevalence of shedding calves was highest at ages 4 and 5 weeks; however, the feces with the highest oocyst per gram (OPG) were registered at 2-4 weeks of age. This is earlier than what has been found in most other studies, indicating that the infection dynamics of C. bovis/C. ryanae may be affected by the presence of C. parvum. The OPG for the positive calves ranged from 200 to 1.1 × 10

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