Development and Validation of a Quantitative UHPLC-MS-MS Method for the Determination of Alpha-Chloralose in Feline Blood and Application on Blood Samples Collected from Cats with Symptoms of Alpha-Chloralose Poisoning.
Journal
Journal of analytical toxicology
ISSN: 1945-2403
Titre abrégé: J Anal Toxicol
Pays: England
ID NLM: 7705085
Informations de publication
Date de publication:
14 Jul 2022
14 Jul 2022
Historique:
received:
26
04
2021
revised:
23
07
2021
accepted:
27
07
2021
pubmed:
28
7
2021
medline:
19
7
2022
entrez:
27
7
2021
Statut:
ppublish
Résumé
Alpha-chloralose (AC) is used as a rodenticide as well as an anesthetic agent in laboratory animals. It was previously also used as an avicide. Detection of AC in blood samples or in body tissues collected postmortem is key for the diagnosis of clinical cases and a requirement for surveillance of secondary toxicosis, including potential cases in wild animals. Reports on poisoning of humans and non-laboratory animals confirmed by the detection of AC or its metabolites are available, however poisoning of domestic animals are rarely available. Furthermore, reports on clinical cases in domestic animals rarely report quantifications of AC in blood or body tissues. The present study describes the validation of a quantitative ultra high performance liquid chromatography--tandem mass spectrometry (UHPLC--MS-MS) method that can be used in cases of suspected AC poisoning in cats. The validation study showed the method to be fit for purpose. In serum, the limit of quantification was 100 ng/mL and the limit of detection was 30 ng/mL. The new analytical method was applied on blood samples collected from 20 individual cats with a preliminary clinical diagnosis of acute AC poisoning. AC was confirmed in all 20 feline blood samples, and the concentration range of AC was 538-17,500 ng/mL. The quantitative method developed in this study was found to be a fast and selective method for confirmation of AC poisoning using blood samples from cats.
Identifiants
pubmed: 34313718
pii: 6329094
doi: 10.1093/jat/bkab087
pmc: PMC9282274
doi:
Substances chimiques
Rodenticides
0
Chloralose
238BZ29MUE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
651-657Subventions
Organisme : Djurvännernas FÖrening, Stockholm, Animal Welfare Sweden
Organisme : The Swedish Environmental Protection Agency
Organisme : Djurvännernas Förening, Stockholm, Animal Welfare Sweden
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press.
Références
J Anal Toxicol. 2016 May;40(4):277-85
pubmed: 26984200
PLoS One. 2013 Sep 09;8(9):e73771
pubmed: 24040064
Analyst. 1984 Oct;109(10):1335-8
pubmed: 6524678
Vet Rec. 1972 Sep 30;91(14):330-3
pubmed: 4562133
Psychopharmacologia. 1964 Jul 6;6(1):1-30
pubmed: 5318644
J Anal Toxicol. 2012 Jul;36(6):452-6
pubmed: 22572811
Eur Neuropsychopharmacol. 2016 Mar;26(3):518-31
pubmed: 26796682
Forensic Sci Int. 1999 Sep 30;104(1):59-63
pubmed: 10533278
Anaesthesist. 2019 Dec;68(12):843-847
pubmed: 31701172
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 15;805(2):303-9
pubmed: 15135105
Hum Toxicol. 1988 May;7(3):285-7
pubmed: 3391628
Vet J. 2006 Jul;172(1):109-13
pubmed: 16772135
Vet Hum Toxicol. 1991 Apr;33(2):161-5
pubmed: 2035246
Br J Pharmacol. 2000 Feb;129(4):731-43
pubmed: 10683198
J Anal Toxicol. 2003 Apr;27(3):156-61
pubmed: 12731657
Int J Legal Med. 1996;108(4):191-3
pubmed: 8652423
Lab Anim Sci. 1987 Oct;37(5):587-95
pubmed: 3320515