The Cisplatin, 5-fluorouracil, Irinotecan, and Gemcitabine Treatment in Resistant 2D and 3D Model Triple Negative Breast Cancer Cell Line: ABCG2 Expression Data.
ATP Binding Cassette Transporter, Subfamily G, Member 2
/ antagonists & inhibitors
Antineoplastic Agents
/ chemistry
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Cisplatin
/ chemistry
Deoxycytidine
/ analogs & derivatives
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Fluorouracil
/ chemistry
Humans
Irinotecan
/ chemistry
Neoplasm Proteins
/ antagonists & inhibitors
Structure-Activity Relationship
Triple Negative Breast Neoplasms
/ drug therapy
Tumor Cells, Cultured
Gemcitabine
3D spheroid formation
5-Fu
Cisplatin
breast cancer
drug resistance
gemcitabine
gene expression
irinotecan
Journal
Anti-cancer agents in medicinal chemistry
ISSN: 1875-5992
Titre abrégé: Anticancer Agents Med Chem
Pays: Netherlands
ID NLM: 101265649
Informations de publication
Date de publication:
2022
2022
Historique:
received:
06
11
2020
revised:
04
05
2021
accepted:
14
06
2021
pubmed:
29
7
2021
medline:
31
3
2022
entrez:
28
7
2021
Statut:
ppublish
Résumé
Chemotherapeutics have been commonly used in cancer treatment. In this study, the effects of Cisplatin, 5-fluorouracil, Irinotecan, and Gemcitabine have been evaluated on two-dimensional (2D) (sensitive and resistance) cell lines and three dimensional (3D) spheroid structure of MDA-MB- 231. The 2D cell culture lacks a natural tissue-like structural so, using 3D cell culture has an important role in the development of effective drug testing models. Furthermore, we analyzed the ATP Binding Cassette Subfamily G Member 2 (ABCG2) gene and protein expression profile in this study. We aimed to establish a 3D breast cancer model that can mimic the in vivo 3D breast cancer microenvironment. The 3D spheroid structures were multiplied (globally) using the three-dimensional hanging drop method. The cultures of the parental cell line MDA-MB-231 served as the controls. After adding the drugs in different amounts, we observed a clear and well-differentiated spheroid formation for 24 h. The viability and proliferation capacity of 2D (sensitive and resistant) cell lines and 3D spheroid cell treatment were assessed by the XTT assay. Cisplatin, Irinotecan, 5-Fu, and Gemcitabine-resistant MDA-MB-231 cells were observed to begin to disintegrate in a three-dimensional clustered structure at 24 hours. Additionally, RT-PCR and protein assay showed overexpression of ABCG2 when compared to the parental cell line. Moreover, MDA-MB-231 cells grown in 3D showed decreased sensitivity to chemotherapeutics treatment. More resistance to chemotherapeutics and altered gene expression profile were shown in 3D cell cultures when compared with the 2D cells. These results might play an important role to evaluate the efficacy of anticancer drugs to explore the mechanisms of MDR in the 3D spheroid forms.
Sections du résumé
BACKGROUND
Chemotherapeutics have been commonly used in cancer treatment.
OBJECTIVE
In this study, the effects of Cisplatin, 5-fluorouracil, Irinotecan, and Gemcitabine have been evaluated on two-dimensional (2D) (sensitive and resistance) cell lines and three dimensional (3D) spheroid structure of MDA-MB- 231. The 2D cell culture lacks a natural tissue-like structural so, using 3D cell culture has an important role in the development of effective drug testing models. Furthermore, we analyzed the ATP Binding Cassette Subfamily G Member 2 (ABCG2) gene and protein expression profile in this study. We aimed to establish a 3D breast cancer model that can mimic the in vivo 3D breast cancer microenvironment.
METHODS
The 3D spheroid structures were multiplied (globally) using the three-dimensional hanging drop method. The cultures of the parental cell line MDA-MB-231 served as the controls. After adding the drugs in different amounts, we observed a clear and well-differentiated spheroid formation for 24 h. The viability and proliferation capacity of 2D (sensitive and resistant) cell lines and 3D spheroid cell treatment were assessed by the XTT assay.
RESULTS
Cisplatin, Irinotecan, 5-Fu, and Gemcitabine-resistant MDA-MB-231 cells were observed to begin to disintegrate in a three-dimensional clustered structure at 24 hours. Additionally, RT-PCR and protein assay showed overexpression of ABCG2 when compared to the parental cell line. Moreover, MDA-MB-231 cells grown in 3D showed decreased sensitivity to chemotherapeutics treatment.
CONCLUSION
More resistance to chemotherapeutics and altered gene expression profile were shown in 3D cell cultures when compared with the 2D cells. These results might play an important role to evaluate the efficacy of anticancer drugs to explore the mechanisms of MDR in the 3D spheroid forms.
Identifiants
pubmed: 34315389
pii: ACAMC-EPUB-116868
doi: 10.2174/1871520621666210727105431
doi:
Substances chimiques
ABCG2 protein, human
0
ATP Binding Cassette Transporter, Subfamily G, Member 2
0
Antineoplastic Agents
0
Neoplasm Proteins
0
Deoxycytidine
0W860991D6
Irinotecan
7673326042
Cisplatin
Q20Q21Q62J
Fluorouracil
U3P01618RT
Gemcitabine
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
371-377Informations de copyright
Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.