Estradiol exerts a neuroprotective effect on SH-SY5Y cells through the miR-106b-5p/TXNIP axis.
SH-SY5Y cell
TXNIP
apoptosis
estradiol
miR-106b-5p
Journal
Journal of biochemical and molecular toxicology
ISSN: 1099-0461
Titre abrégé: J Biochem Mol Toxicol
Pays: United States
ID NLM: 9717231
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
31
03
2021
received:
15
09
2020
accepted:
14
07
2021
pubmed:
29
7
2021
medline:
30
9
2021
entrez:
28
7
2021
Statut:
ppublish
Résumé
Alzheimer's disease (AD) is a neurodegenerative disease. Thioredoxin and thioredoxin-interacting protein (TXNIP) complexes help sustain cell oxidation/reduction balance. In the present study, we verified the neuroprotective role of estradiol against amyloid-beta 42 in SH-SY5Y cells through inhibiting TXNIP expression, promoting cell viability and DNA synthesis ability, inhibiting cell apoptosis, and affecting caspase and Bax/Bcl-2 apoptotic signaling. miR-106b-5p could bind to TXNIP 3'-untranslated region to inhibit the expression level of TXNIP. Within SH-SY5Y cells, miR-106b-5p inhibition repressed cell viability and DNA synthesis ability and promoted cell apoptosis through caspase and Bax/Bcl-2 apoptotic signaling, while miR-106b-5p overexpression or TXNIP knockdown exerted the opposite effects on SH-SY5Y cells; TXNIP knockdown remarkably attenuated the roles of miR-106b-5p inhibition. In conclusion, estradiol treatment on SH-SY5Y cells downregulates TXNIP expression and upregulates miR-106b-5p expression. miR-106b-5p exerts a neuroprotective effect on SH-SY5Y cells by promoting cell proliferation and inhibiting cell apoptosis through targeting TXNIP.
Substances chimiques
Carrier Proteins
0
MIRN106 microRNA, human
0
MicroRNAs
0
Neuroprotective Agents
0
TXNIP protein, human
0
Estradiol
4TI98Z838E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e22861Subventions
Organisme : National Natural Science Foundation of China
ID : 81671398
Organisme : Natural Science Foundation of Hunan Province, China
ID : 2019JJ50912
Organisme : Science and Technology Plan Project of Changsha City
ID : kq2004142
Informations de copyright
© 2021 Wiley Periodicals LLC.
Références
R. A. Armstrong, Folia Neuropathol. 2009, 47, 289.
A. Mietelska-Porowska, U. Wasik, M. Goras, A. Filipek, G. Niewiadomska, Int. J. Mol. Sci. 2014, 15, 4671.
D. J. Selkoe, J. Hardy, EMBO Mol. Med. 2016, 8, 595.
J. Hwang, H. W. Suh, Y. H. Jeon, E. Hwang, L. T. Nguyen, J. Yeom, S. G. Lee, C. Lee, K. J. Kim, B. S. Kang, J. O. Jeong, T. K. Oh, I. Choi, J. O. Lee, M. H. Kim, Nat. Commun. 2014, 5, 2958.
E. Yoshihara, S. Masaki, Y. Matsuo, Z. Chen, H. Tian, J. Yodoi, Front. Immunol. 2014, 4, 514.
S. Akterin, R. F. Cowburn, A. Miranda-Vizuete, A. Jimenez, N. Bogdanovic, B. Winblad, A. Cedazo-Minguez, Cell Death Differ. 2006, 13, 1454.
M. Hokama, S. Oka, J. Leon, T. Ninomiya, H. Honda, K. Sasaki, T. Iwaki, T. Ohara, T. Sasaki, F. M. LaFerla, Y. Kiyohara, Y. Nakabeppu, Cereb. Cortex 2014, 24, 2476.
A. Valavanidis, T. Vlachogianni, C. Fiotakis, J. Environ. Sci. Health, Part C: Toxicol. Carcinog. 2009, 27, 120.
C. Zhang, G. Nestorova, R. A. Rissman, J. Feng, Electrophoresis 2013, 34, 2268.
C. Y. Wang, Y. Xu, X. Wang, C. Guo, T. Wang, Z. Y. Wang, Antioxid. Redox Signaling 2019, 30, 1411.
Q. Pan, K. Guo, Y. Li, Q. Tu, Zhongnan Daxue Xuebao, Yixueban 2019, 44, 1360.
Q. Pan, K. Guo, M. Xue, Q. Tu, Neurochem. Int. 2020, 135, 104685.
A. L. Svensson, A. Nordberg, NeuroReport 1999, 10, 3485.
O. Y. Bang, H. S. Hong, D. H. Kim, H. Kim, J. H. Boo, K. Huh, I. Mook-Jung, Neurobiol. Dis. 2004, 16, 21.
M. Alvarez-de-la-Rosa, I. Silva, J. Nilsen, M. M. Perez, L. M. Garcia-Segura, J. Avila, F. Naftolin, Ann. N. Y. Acad. Sci. 2005, 1052, 210.
L. I. Binder, A. Frankfurter, L. I. Rebhun, J. Cell Biol. 1985, 101, 1371.
S. C. Papasozomenos, L. I. Binder, Cell Motil. Cytoskeleton 1987, 8, 210.
V. Ambros, Nature 2004, 431(7006), 350.
O. Barad, E. Meiri, A. Avniel, R. Aharonov, A. Barzilai, I. Bentwich, U. Einav, S. Gilad, P. Hurban, Y. Karov, E. K. Lobenhofer, E. Sharon, Y. M. Shiboleth, M. Shtutman, Z. Bentwich, P. Einat, Genome Res. 2004, 14, 2486.
E. A. Miska, E. Alvarez-Saavedra, M. Townsend, A. Yoshii, N. Sestan, P. Rakic, M. Constantine-Paton, H. R. Horvitz, Genome Biol. 2004, 5, R68.
L. F. Sempere, S. Freemantle, I. Pitha-Rowe, E. Moss, E. Dmitrovsky, V. Ambros, Genome Biol. 2004, 5, R13.
S. S. Hebert, B. De Strooper, Science 2007, 317, 1179.
S. S. Hebert, K. Horre, L. Nicolai, A. S. Papadopoulou, W. Mandemakers, A. N. Silahtaroglu, S. Kauppinen, A. Delacourte, B. De Strooper, Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 6415.
W. X. Wang, B. W. Rajeev, A. J. Stromberg, N. Ren, G. Tang, Q. Huang, I. Rigoutsos, P. T. Nelson, J. Neurosci. 2008, 28(5), 1213.
S. S. Hebert, K. Horre, L. Nicolai, B. Bergmans, A. S. Papadopoulou, A. Delacourte, B. De Strooper, Neurobiol. Dis. 2009, 33, 422.
W. Liu, J. Zhao, G. Lu, Biochem. Biophys. Res. Commun. 2016, 478, 852.
H. Wang, L. Yuan, W. Ma, J. Han, Y. Lu, L. Feng, R. Xiao, Neurosci. Lett. 2016, 632, 209.
Z. Liu, C. Dou, B. Yao, M. Xu, L. Ding, Y. Wang, Y. Jia, Q. Li, H. Zhang, K. Tu, T. Song, Q. Liu, Oncotarget 2016, 7, 25350.
K. Tu, Z. Liu, B. Yao, S. Han, W. Yang, Int. J. Oncol. 2016, 48, 965.
B. J. Deroo, K. S. Korach, J. Clin. Invest. 2006, 116, 561.
M. Jia, K. Dahlman-Wright, J. A. Gustafsson, Best Pract. Res., Clin. Endocrinol. Metab. 2015, 29, 557.
S. Tang, Y. Ren, X. Ren, J. Cao, H. Hong, H. Ji, Q. Hu, Exp. Neurol. 2019, 311, 33.
J. W. Park, S. H. Lee, G. H. Woo, H. J. Kwon, D. Y. Kim, Biochem. Biophys. Res. Commun. 2018, 498, 566.
X. F. Lan, X. J. Zhang, Y. N. Lin, Q. Wang, H. J. Xu, L. N. Zhou, P. L. Chen, Q. Y. Li, Sci. Rep. 2017, 7, 10318.
H. K. Karnati, M. K. Panigrahi, R. K. Gutti, N. H. Greig, I. A. Tamargo, J. Alzheimer's Dis. 2015, 48, 563.
J. Nunez-Iglesias, C. C. Liu, T. E. Morgan, C. E. Finch, X. J. Zhou, PLoS One 2010, 5, e8898.
O. C. Maes, H. M. Chertkow, E. Wang, H. M. Schipper, Curr. Genomics 2009, 10, 154.
G. Lee, R. Thangavel, V. M. Sharma, J. M. Litersky, K. Bhaskar, S. M. Fang, L. H. Do, A. Andreadis, G. Van Hoesen, H. Ksiezak-Reding, J. Neurosci. 2004, 24, 2304.
M. Larson, M. A. Sherman, F. Amar, M. Nuvolone, J. A. Schneider, D. A. Bennett, A. Aguzzi, S. E. Lesne, J. Neurosci. 2012, 32, 16857.
J. W. Um, H. B. Nygaard, J. K. Heiss, M. A. Kostylev, M. Stagi, A. Vortmeyer, T. Wisniewski, E. C. Gunther, S. M. Strittmatter, Nat. Neurosci. 2012, 15, 1227.
X. Quan, H. Liang, Y. Chen, Q. Qin, Y. Wei, Z. Liang, Med. Sci. Monit. 2020, 26, e919311.
A. Rovelet-Lecrux, D. Hannequin, G. Raux, N. Le Meur, A. Laquerrière, A. Vital, C. Dumanchin, S. Feuillette, A. Brice, M. Vercelletto, F. Dubas, T. Frebourg, D. Campion, Nat. Genet. 2006, 38(1), 24.
J. Theuns, N. Brouwers, S. Engelborghs, K. Sleegers, V. Bogaerts, E. Corsmit, T. De Pooter, C. M. van Duijn, P. P. De Deyn, C. Van Broeckhoven, Am. J. Hum. Genet. 2006, 78(6), 936.
S. Nasoohi, K. Parveen, T. Ishrat, J. Alzheimer's Dis. 2018, 66, 857.