Microchip Electrophoresis Assay for Calmodulin Binding Proteins.
calmodulin binding protein
colloidal array
micellar electrokinetic chromatography
microchip electrophoresis
photo-crosslinking
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
entrez:
29
7
2021
pubmed:
30
7
2021
medline:
15
12
2021
Statut:
ppublish
Résumé
The calcium signaling protein calmodulin regulates numerous intracellular processes. We introduce a sensitive microchip assay to separate and detect calmodulin binding proteins. The assay utilizes an optimized microchip electrophoresis protein separation platform with laser-induced fluorescence detection. Fluorescence-labeled calmodulin modified with a photoreactive diazirine crosslinker allowed selective detection of calmodulin binding proteins. We demonstrate successful in
Identifiants
pubmed: 34321981
doi: 10.1002/jssc.202000884
pmc: PMC8312402
mid: NIHMS1690303
doi:
Substances chimiques
Calmodulin-Binding Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
895-902Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103638
Pays : United States
Déclaration de conflit d'intérêts
Conflict of interest statement The authors declare no financial/commercial conflicts of interest.
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