Imaging multiple sclerosis pathology at 160 μm isotropic resolution by human whole-brain ex vivo magnetic resonance imaging at 3 T.
Aged
Biomedical Engineering
Brain
/ diagnostic imaging
Humans
Imaging, Three-Dimensional
Immunohistochemistry
Magnetic Resonance Imaging
/ methods
Male
Middle Aged
Multiple Sclerosis
/ diagnostic imaging
Neuroimaging
/ methods
Reproducibility of Results
Signal-To-Noise Ratio
Thalamus
/ diagnostic imaging
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 07 2021
29 07 2021
Historique:
received:
07
01
2021
accepted:
13
07
2021
entrez:
30
7
2021
pubmed:
31
7
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Postmortem magnetic resonance imaging (MRI) of the fixed healthy and diseased human brain facilitates spatial resolutions and image quality that is not achievable with in vivo MRI scans. Though challenging-and almost exclusively performed at 7 T field strength-depicting the tissue architecture of the entire brain in fine detail is invaluable since it enables the study of neuroanatomy and uncovers important pathological features in neurological disorders. The objectives of the present work were (1) to develop a 3D isotropic ultra-high-resolution imaging approach for human whole-brain ex vivo acquisitions working on a standard clinical 3 T MRI system; and (2) to explore the sensitivity and specificity of this concept for specific pathoanatomical features of multiple sclerosis. The reconstructed images demonstrate unprecedented resolution and soft tissue contrast of the diseased human brain at 3 T, thus allowing visualization of sub-millimetric lesions in the different cortical layers and in the cerebellar cortex, as well as unique cortical lesion characteristics such as the presence of incomplete/complete iron rims, and patterns of iron accumulation. Further details such as the subpial molecular layer, the line of Gennari, and some intrathalamic nuclei are also well distinguishable.
Identifiants
pubmed: 34326420
doi: 10.1038/s41598-021-94891-1
pii: 10.1038/s41598-021-94891-1
pmc: PMC8322069
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15491Informations de copyright
© 2021. The Author(s).
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