The two Pseudomonas aeruginosa DksA stringent response proteins are largely interchangeable at the whole transcriptome level and in the control of virulence-related traits.


Journal

Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692

Informations de publication

Date de publication:
09 2021
Historique:
revised: 22 07 2021
received: 19 04 2021
accepted: 27 07 2021
pubmed: 31 7 2021
medline: 15 12 2021
entrez: 30 7 2021
Statut: ppublish

Résumé

The stringent response regulator DksA plays a key role in Gram negative bacteria adaptation to challenging environments. Intriguingly, the plant and human pathogen Pseudomonas aeruginosa is unique as it expresses two functional DksA paralogs: DksA1 and DksA2. However, the role of DksA2 in P. aeruginosa adaptive strategies has been poorly investigated so far. Here, RNA-Seq analysis and phenotypic assays showed that P. aeruginosa DksA1 and DksA2 proteins are largely interchangeable. Relative to wild type P. aeruginosa, transcription of 1779 genes was altered in a dksA1 dksA2 double mutant, and the wild type expression level of ≥90% of these genes was restored by in trans complementation with either dksA1 or dksA2. Interestingly, the expression of a small sub-set of genes seems to be preferentially or exclusively complemented by either dksA1 or dksA2. In addition, evidence has been provided that the DksA-dependent regulation of virulence genes expression is independent and hierarchically dominant over two major P. aeruginosa regulatory circuits, i.e., quorum sensing and cyclic-di-GMP signalling systems. Our findings support the prominent role of both DksA paralogs in P. aeruginosa environmental adaptation.

Identifiants

pubmed: 34327807
doi: 10.1111/1462-2920.15693
doi:

Substances chimiques

Bacterial Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5487-5504

Informations de copyright

© 2021 Society for Applied Microbiology and John Wiley & Sons Ltd.

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Auteurs

Alessandra Fortuna (A)

Department of Science, University Roma Tre, Rome, Italy.

Heike Bähre (H)

Research Core Unit Metabolomics, Hannover Medical School, Hannover, Germany.

Paolo Visca (P)

Department of Science, University Roma Tre, Rome, Italy.

Giordano Rampioni (G)

Department of Science, University Roma Tre, Rome, Italy.

Livia Leoni (L)

Department of Science, University Roma Tre, Rome, Italy.

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