A magnified aptamer fluorescence sensor based on the metal organic frameworks adsorbed DNA with enzyme catalysis amplification for ultra-sensitive determination of ATP and its logic gate operation.
Adenosine Triphosphate
/ blood
Adsorption
Animals
Aptamers, Nucleotide
/ chemistry
Biosensing Techniques
/ methods
Cattle
Chromium
/ chemistry
Exodeoxyribonucleases
/ chemistry
Fluoresceins
/ chemistry
Fluorescence
Fluorescent Dyes
/ chemistry
Limit of Detection
Logic
Metal-Organic Frameworks
/ chemistry
Adenosine triphosphate
Aptamer
DNA
Enzyme catalysis
Fluorescent sensor
Logic gate
Metal organic frameworks
Signal amplification
Journal
Bioorganic chemistry
ISSN: 1090-2120
Titre abrégé: Bioorg Chem
Pays: United States
ID NLM: 1303703
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
10
02
2021
revised:
13
04
2021
accepted:
22
05
2021
pubmed:
31
7
2021
medline:
16
12
2021
entrez:
30
7
2021
Statut:
ppublish
Résumé
With the development of frame materials, metal organic frameworks (MOFs) have been successfully applied in the fields of biological small molecule analysis and fluorescent DNA detection. In this work, in view of the good adsorption characteristics of MIL-101(Cr), the highly sensitive detection of adenosine triphosphate (ATP) assisted nucleic acid exonuclease amplification by MIL-101(Cr) on the different affinity of single stranded DNA and double stranded DNA was investigated. The detection limit of ATP reaches 1.7 μM, and the platform has good applicability in biological samples. On this basis, an "AND" logic gate was successfully constructed. Superior sensitivity to ATP in the presence of exonuclease was reflected, which greatly enhanced the system's fluorescence. Importantly, the fluorescence sensing application of this nanomaterial inspired other target detection and enriched the building blocks of fluorescence sensing platform.
Identifiants
pubmed: 34328850
pii: S0045-2068(21)00397-7
doi: 10.1016/j.bioorg.2021.105020
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Fluoresceins
0
Fluorescent Dyes
0
MIL-101
0
Metal-Organic Frameworks
0
Chromium
0R0008Q3JB
Adenosine Triphosphate
8L70Q75FXE
Exodeoxyribonucleases
EC 3.1.-
exodeoxyribonuclease III
EC 3.1.11.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105020Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.