Bioprinting of dual ECM scaffolds encapsulating limbal stem/progenitor cells in active and quiescent statuses.
DLP-based bioprinting
endogenous stem cell
hyaluronic acid
limbal stem cell
regenerative medicine
stem cell quiescence
Journal
Biofabrication
ISSN: 1758-5090
Titre abrégé: Biofabrication
Pays: England
ID NLM: 101521964
Informations de publication
Date de publication:
13 08 2021
13 08 2021
Historique:
received:
20
01
2021
accepted:
30
07
2021
pubmed:
31
7
2021
medline:
26
11
2021
entrez:
30
7
2021
Statut:
epublish
Résumé
Limbal stem cell deficiency and corneal disorders are among the top global threats for human vision. Emerging therapies that integrate stem cell transplantation with engineered hydrogel scaffolds for biological and mechanical support are becoming a rising trend in the field. However, methods for high-throughput fabrication of hydrogel scaffolds, as well as knowledge of the interaction between limbal stem/progenitor cells (LSCs) and the surrounding extracellular matrix (ECM) are still much needed. Here, we employed digital light processing (DLP)-based bioprinting to fabricate hydrogel scaffolds encapsulating primary LSCs and studied the ECM-dependent LSC phenotypes. The DLP-based bioprinting with gelatin methacrylate (GelMA) or hyaluronic acid glycidyl methacrylate (HAGM) generated microscale hydrogel scaffolds that could support the viability of the encapsulated primary rabbit LSCs (rbLSCs) in culture. Immunocytochemistry and transcriptional analysis showed that the encapsulated rbLSCs remained active in GelMA-based scaffolds while exhibited quiescence in the HAGM-based scaffolds. The primary human LSCs encapsulated within bioprinted scaffolds showed consistent ECM-dependent active/quiescent statuses. Based on these results, we have developed a novel bioprinted dual ECM 'Yin-Yang' model encapsulating LSCs to support both active and quiescent statues. Our findings provide valuable insights towards stem cell therapies and regenerative medicine for corneal reconstruction.
Identifiants
pubmed: 34330126
doi: 10.1088/1758-5090/ac1992
pmc: PMC8716326
mid: NIHMS1764798
doi:
Substances chimiques
Gelatin
9000-70-8
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NEI NIH HHS
ID : R01 EY021797
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY028557
Pays : United States
Organisme : NEI NIH HHS
ID : R21 EY031122
Pays : United States
Informations de copyright
© 2021 IOP Publishing Ltd.
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