Gene signatures of autopsy lungs from obese patients with COVID-19.


Journal

Clinical nutrition ESPEN
ISSN: 2405-4577
Titre abrégé: Clin Nutr ESPEN
Pays: England
ID NLM: 101654592

Informations de publication

Date de publication:
08 2021
Historique:
received: 29 04 2021
accepted: 05 05 2021
entrez: 31 7 2021
pubmed: 1 8 2021
medline: 17 8 2021
Statut: ppublish

Résumé

Obesity is associated with low grade systemic inflammation and insulin resistance. Although metabolic and immunological changes may contribute to the increased risk for COVID-19 mortality in obese, little is known about the impact of obesity in the lungs of patients with COVID-19. We analyzed gene expression profiles of autopsy lungs of a cohort of 14 COVID-19 patients and 4 control individuals. Patients were divided into 3 groups according to their comorbidities: hypertension, type 2 diabetes (T2D) and obesity. We then identified the molecular alterations associated with these comorbidities in COVID-19 patients. Patients with only hypertension showed higher levels of inflammatory genes and B-cell related genes when compared to those with T2D and obesity. However, the levels of IFN-gamma, IL22, and CD274 (a ligand that binds to receptor PD1) were higher in COVID-19 patients with T2D and obesity. Several metabolic- and immune-associated genes such as G6PD, LCK and IL10 were significantly induced in the lungs of the obese group. Our findings suggest that SARS-CoV-2 infection in the lungs may exacerbate the immune response and chronic condition in obese COVID-19 patients.

Sections du résumé

BACKGROUND & AIMS
Obesity is associated with low grade systemic inflammation and insulin resistance. Although metabolic and immunological changes may contribute to the increased risk for COVID-19 mortality in obese, little is known about the impact of obesity in the lungs of patients with COVID-19.
METHODS
We analyzed gene expression profiles of autopsy lungs of a cohort of 14 COVID-19 patients and 4 control individuals. Patients were divided into 3 groups according to their comorbidities: hypertension, type 2 diabetes (T2D) and obesity. We then identified the molecular alterations associated with these comorbidities in COVID-19 patients.
RESULTS
Patients with only hypertension showed higher levels of inflammatory genes and B-cell related genes when compared to those with T2D and obesity. However, the levels of IFN-gamma, IL22, and CD274 (a ligand that binds to receptor PD1) were higher in COVID-19 patients with T2D and obesity. Several metabolic- and immune-associated genes such as G6PD, LCK and IL10 were significantly induced in the lungs of the obese group.
CONCLUSION
Our findings suggest that SARS-CoV-2 infection in the lungs may exacerbate the immune response and chronic condition in obese COVID-19 patients.

Identifiants

pubmed: 34330510
pii: S2405-4577(21)00184-4
doi: 10.1016/j.clnesp.2021.05.004
pmc: PMC8149170
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

475-478

Informations de copyright

Copyright © 2021 European Society for Clinical Nutrition and Metabolism. Published by Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest None of the authors have conflicts of interest to disclose.

Références

Front Immunol. 2020 Aug 18;11:2063
pubmed: 33013872
Chest. 2021 Jan;159(1):239-248
pubmed: 32795478
Aging Dis. 2019 Dec 1;10(6):1174-1186
pubmed: 31788330
J Leukoc Biol. 2013 Apr;93(4):561-71
pubmed: 23341537
Nat Commun. 2020 Oct 8;11(1):5086
pubmed: 33033248
Obesity (Silver Spring). 2020 Jul;28(7):1195-1199
pubmed: 32271993

Auteurs

Juan Carlo Santos E Silva (JC)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.

Amanda Pereira Vasconcelos (AP)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.

Isabella Harumi Yonehara Noma (IHY)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.

Natália Yumi Noronha (NY)

Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, Brazil.

Rodrigo Aquino (R)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.

Jeevan Giddaluru (J)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Scientific Platform Pasteur-University of São Paulo, São Paulo, Brazil.

Luiz Durão (L)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.

André Guilherme Costa-Martins (AG)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Scientific Platform Pasteur-University of São Paulo, São Paulo, Brazil.

Viviane Schuch (V)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.

Pedro M Moraes-Vieira (PM)

Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Brazil; Obesity and Comorbidities Research Center (OCRC), University of Campinas, Campinas, São Paulo, Brazil.

Helder I Nakaya (HI)

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Scientific Platform Pasteur-University of São Paulo, São Paulo, Brazil. Electronic address: hnakaya@usp.br.

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Classifications MeSH