Preparation and Identification of Cardiac Myofibrils from Whole Heart Samples.
Cardiac myofibrils
Mass spectrometry
Mouse
Protein separation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
31
7
2021
pubmed:
1
8
2021
medline:
17
8
2021
Statut:
ppublish
Résumé
Mouse models are extensively studied and well-used to study cardiomyopathies due to the genetic relevance of this model organism and increasing need to identify therapeutic targets. Cardiac myofibril preparation for whole hearts has proved to be an invaluable in vitro method for determining the fundamental molecular mechanisms in heart failure. The technique described below consistently yields intact cardiac myofibrils, which can be used in subsequent techniques such as western blotting, immunofluorescence, and mass spectrometry. Here, we describe a method to optimize the separation and yield of cardiac myofibrils from murine whole tissue samples and preparation for subsequent mass spectrometry. This method allows for quick visual identification of multiple cardiac myofibril proteins.
Identifiants
pubmed: 34331238
doi: 10.1007/978-1-0716-1480-8_2
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
15-24Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL145534
Pays : United States
Informations de copyright
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.
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