Versatile On-Demand Fluorescent Labeling of Fusion Proteins Using Fluorescence-Activating and Absorption-Shifting Tag (FAST).
Fluorescence labeling
Fluorogenic chromophore
Non-covalent labeling
Protein tag
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
31
7
2021
pubmed:
1
8
2021
medline:
2
9
2021
Statut:
ppublish
Résumé
Observing the localization, the concentration, and the distribution of proteins in cells or organisms is essential to understand theirs functions. General and versatile methods allowing multiplexed imaging of proteins under a large variety of experimental conditions are thus essential for deciphering the inner workings of cells and organisms. Here, we present a general method based on the non-covalent labeling of a small protein tag, named FAST (fluorescence-activating and absorption-shifting tag), with various fluorogenic ligands that light up upon labeling, which makes the simple, robust, and versatile on-demand labeling of fusion proteins in a wide range of experimental systems possible.
Identifiants
pubmed: 34331290
doi: 10.1007/978-1-0716-1593-5_16
doi:
Substances chimiques
Fluorescent Dyes
0
Recombinant Fusion Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
253-265Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.
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