Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis.


Journal

BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551

Informations de publication

Date de publication:
31 Jul 2021
Historique:
received: 19 03 2021
accepted: 05 07 2021
entrez: 1 8 2021
pubmed: 2 8 2021
medline: 25 9 2021
Statut: epublish

Résumé

Human intestinal spirochetosis (HIS) is an infectious disease of large intestines caused by Brachyspira species, and most HIS cases are asymptomatic or exhibit mild intestinal symptoms. The host reaction to HIS remains unclear, and we examined HIS-related mucosal inflammatory features histologically. From the archival HIS cases in a single medical center, 24 endoscopically taken specimens from 14 HIS cases (male:female = 10:4; 28-73 yrs) were selected as not containing polypoid or neoplastic lesions. Stromal neutrophils, eosinophils, and mast cells, and intraepithelial neutrophils and eosinophils, (sNeu, sEo, sMast, iNeu, and iEo, respectively) were counted, and the presence or absence of lymphoid follicles/aggregates (LFs) was also examined. Association of the above inflammation parameters and spirochetal infection parameters (such as degrees of characteristic fringe distribution, of spirochetal cryptal invasion, and of spirochetal intraepithelial invasion) were also analysed. iNeu was observed in 29.2%, iEo in 58.3%, and LFs in 50.0% of the specimens. Maximal counts of sNeu, sEo, sMast, iNeu, and iEo averaged 8.4, 21.5, 6.0, 0.5 and 1.5, respectively. Strong correlation between the maximum counts of iNeu and iEo (p < 0.001, r = 0.81), and correlations between those of iEo and sNeu (p = 0.0012, r = 0.62) and between those of iEo and sEo (p = 0.026, r = 0.45) were observed. iNeu was influenced by fringe formation (p < 0.05) and spirochetal crypt involvement (p < 0.05). HIS was accompanied by inflammatory reactions, and among these, mucosal eosinophilic infiltration may be a central indicator and host reaction of HIS.

Sections du résumé

BACKGROUND BACKGROUND
Human intestinal spirochetosis (HIS) is an infectious disease of large intestines caused by Brachyspira species, and most HIS cases are asymptomatic or exhibit mild intestinal symptoms. The host reaction to HIS remains unclear, and we examined HIS-related mucosal inflammatory features histologically.
METHODS METHODS
From the archival HIS cases in a single medical center, 24 endoscopically taken specimens from 14 HIS cases (male:female = 10:4; 28-73 yrs) were selected as not containing polypoid or neoplastic lesions. Stromal neutrophils, eosinophils, and mast cells, and intraepithelial neutrophils and eosinophils, (sNeu, sEo, sMast, iNeu, and iEo, respectively) were counted, and the presence or absence of lymphoid follicles/aggregates (LFs) was also examined. Association of the above inflammation parameters and spirochetal infection parameters (such as degrees of characteristic fringe distribution, of spirochetal cryptal invasion, and of spirochetal intraepithelial invasion) were also analysed.
RESULTS RESULTS
iNeu was observed in 29.2%, iEo in 58.3%, and LFs in 50.0% of the specimens. Maximal counts of sNeu, sEo, sMast, iNeu, and iEo averaged 8.4, 21.5, 6.0, 0.5 and 1.5, respectively. Strong correlation between the maximum counts of iNeu and iEo (p < 0.001, r = 0.81), and correlations between those of iEo and sNeu (p = 0.0012, r = 0.62) and between those of iEo and sEo (p = 0.026, r = 0.45) were observed. iNeu was influenced by fringe formation (p < 0.05) and spirochetal crypt involvement (p < 0.05).
CONCLUSIONS CONCLUSIONS
HIS was accompanied by inflammatory reactions, and among these, mucosal eosinophilic infiltration may be a central indicator and host reaction of HIS.

Identifiants

pubmed: 34332545
doi: 10.1186/s12879-021-06418-8
pii: 10.1186/s12879-021-06418-8
pmc: PMC8325824
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

721

Subventions

Organisme : Japan Society for the Promotion of Science
ID : JP 18K07034

Informations de copyright

© 2021. The Author(s).

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Auteurs

Sho Ogata (S)

Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa, Saitama, 359-8513, Japan. sogata@ndmc.ac.jp.
Department of Diagnostic Pathology, JCHO Saitama Medical Center, Saitama, Saitama, 330-0074, Japan. sogata@ndmc.ac.jp.

Ken Shimizu (K)

Department of Diagnostic Pathology, JCHO Saitama Medical Center, Saitama, Saitama, 330-0074, Japan.

Susumu Tominaga (S)

Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa, Saitama, 359-8513, Japan.

Susumu Matsukuma (S)

Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa, Saitama, 359-8513, Japan.
Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Saitama, 359-8513, Japan.

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Classifications MeSH