The Conditioned Medium of Lactobacillus rhamnoides GG Regulates Microglia/Macrophage Polarization and Improves Functional Recovery after Spinal Cord Injury in Rats.
Animals
Cell Polarity
/ drug effects
Cell Survival
/ drug effects
Culture Media, Conditioned
/ pharmacology
Female
Inflammation
/ pathology
Lacticaseibacillus rhamnosus
/ chemistry
Macrophages
/ drug effects
Microglia
/ drug effects
Motor Activity
/ drug effects
NF-KappaB Inhibitor alpha
/ metabolism
NF-kappa B
/ metabolism
Phosphorylation
/ drug effects
Rats
Recovery of Function
/ drug effects
Signal Transduction
/ drug effects
Spinal Cord Injuries
/ physiopathology
Journal
BioMed research international
ISSN: 2314-6141
Titre abrégé: Biomed Res Int
Pays: United States
ID NLM: 101600173
Informations de publication
Date de publication:
2021
2021
Historique:
received:
07
04
2021
accepted:
25
06
2021
entrez:
2
8
2021
pubmed:
3
8
2021
medline:
22
9
2021
Statut:
epublish
Résumé
Lactobacillus rhamnoides, a human intestinal colonizer, can act through various pathways to induce microglia/macrophages to produce cytokines and to polarize microglia/macrophages to different phenotypes to reduce the inflammatory response. In this article, we evaluated the treatment potential of the Lactobacillus rhamnoides GG conditioned medium (LGG-CM) in rat model with SCI (acute spinal cord injury), including functional, neurophysiological, and histological outcomes and the underlying neuroprotective mechanisms. In our experiment, LGG-CM (30 mg/kg) was injected directly into the injury site in rats immediately after SCI. Measured by the BBB scale (Basso, Beattie, and Bresnahan locomotor rating scale) and inclined plane test, rats in the LGG-CM-treated group showed better locomotor scores. Moreover, compared to the vehicle treatment group, LGG-CM increased the mRNA level of the M2 marker (CD206), and decreased that of the M1 marker (iNOS). Western blot assays showed that LGG-CM-treated SCI rats had a higher grayscale ratio of p65 and a lower ratio of p-I
Identifiants
pubmed: 34337004
doi: 10.1155/2021/3376496
pmc: PMC8289592
doi:
Substances chimiques
Culture Media, Conditioned
0
NF-kappa B
0
NF-KappaB Inhibitor alpha
139874-52-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3376496Informations de copyright
Copyright © 2021 Fangqi Lin et al.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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