Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells.
annulus fibrosus
disc degeneration
inorganic polyphosphate
intervertebral disc
proteoglycan matrix
Journal
JOR spine
ISSN: 2572-1143
Titre abrégé: JOR Spine
Pays: United States
ID NLM: 101722350
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
21
07
2020
revised:
20
01
2021
accepted:
13
02
2021
entrez:
2
8
2021
pubmed:
3
8
2021
medline:
3
8
2021
Statut:
epublish
Résumé
Ubiquitously found in all life forms, inorganic polyphosphates (polyP) are linear polymers of repeated orthophosphate units. Present in intervertebral disc tissue, polyP was previously shown to increase extracellular matrix production in nucleus pulposus (NP) cells. However, the effects of polyP on human annulus fibrosus (hAF) cell metabolism is not known. Here, hAF cells cultured in the presence of 0.5 to 1 mM polyP, chain length 22 (polyP-22), showed an increase in glycosaminoglycan content, proteoglycan and collagen synthesis, and aggrecan and collagen type 1 gene expression. Gene expression level of matrix metalloproteinases 1 was reduced while matrix metalloproteinases 3 level was increased in hAF cells treated with 1 mM polyP. Adenosine triphosphate (ATP) synthesis was also significantly increased in hAF cell culture 72 hours after the exposure to 1 mM polyP-22. PolyP thus has both anabolic and bioenergetic effects in AF cells, similar to that observed in NP cells. Together, these results suggest polyP as a potential energy source and a metabolic regulator of disc cells.
Identifiants
pubmed: 34337332
doi: 10.1002/jsp2.1143
pii: JSP21143
pmc: PMC8313173
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e1143Informations de copyright
© 2021 The Authors. JOR Spine published by Wiley Periodicals LLC on behalf of Orthopaedic Research Society.
Déclaration de conflit d'intérêts
The authors declare no competing conflicts of interest.
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