The predominance and diagnostic value of neutrophils in differentiating transudates and exudates in dogs.
canine
mononuclear cells
peritoneal effusion
pleural effusion
septic effusion
Journal
Veterinary clinical pathology
ISSN: 1939-165X
Titre abrégé: Vet Clin Pathol
Pays: United States
ID NLM: 9880575
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
13
11
2020
received:
05
06
2020
accepted:
15
12
2020
pubmed:
3
8
2021
medline:
1
10
2021
entrez:
2
8
2021
Statut:
ppublish
Résumé
There is disagreement in the literature about the proportion of neutrophils expected in canine transudates. A cutoff of <30% neutrophils has been recommended for distinguishing transudates from exudates, but its validity has not been established. The aim of this study was to evaluate differential cell counts in canine effusions and analyze the percentage and number of neutrophils in transudates and exudates. Effusion data were obtained retrospectively from 263 dogs with pleural or peritoneal effusion. Low-protein transudates, high-protein transudates, and exudates were classified using the total protein (TP) concentration and total nucleated cell count (TNCC). Differential percentages and absolute neutrophil counts were compared by the effusion type and underlying etiology. Low-protein transudates (n = 63), high-protein transudates (n = 84), and exudates (n = 77) had a median (range) of 35% (0%-100%), 59% (0%-100%), and 90% (50%-98%) neutrophils (P < .0001). All effusions with <50% neutrophils were transudates, but 53% of transudates had ≥50% neutrophils, and 69% had ≥30%. Median neutrophil counts were 62/µL (0-892/µL), 538/µL (0-4550/µL), and 45 590/µL (5400-496 800/µL) in low-protein transudates, high-protein transudates, and exudates, respectively (P < .0001). Neutrophil counts correlated with TNCC (r Although effusions with <50% neutrophils are transudates, most transudates and exudates have ≥50% neutrophils, limiting the diagnostic usefulness of % neutrophils for classifying effusions. Absolute neutrophil cutoffs did not notably improve effusion classification but could warrant future studies.
Sections du résumé
BACKGROUND
BACKGROUND
There is disagreement in the literature about the proportion of neutrophils expected in canine transudates. A cutoff of <30% neutrophils has been recommended for distinguishing transudates from exudates, but its validity has not been established.
OBJECTIVE
OBJECTIVE
The aim of this study was to evaluate differential cell counts in canine effusions and analyze the percentage and number of neutrophils in transudates and exudates.
METHODS
METHODS
Effusion data were obtained retrospectively from 263 dogs with pleural or peritoneal effusion. Low-protein transudates, high-protein transudates, and exudates were classified using the total protein (TP) concentration and total nucleated cell count (TNCC). Differential percentages and absolute neutrophil counts were compared by the effusion type and underlying etiology.
RESULTS
RESULTS
Low-protein transudates (n = 63), high-protein transudates (n = 84), and exudates (n = 77) had a median (range) of 35% (0%-100%), 59% (0%-100%), and 90% (50%-98%) neutrophils (P < .0001). All effusions with <50% neutrophils were transudates, but 53% of transudates had ≥50% neutrophils, and 69% had ≥30%. Median neutrophil counts were 62/µL (0-892/µL), 538/µL (0-4550/µL), and 45 590/µL (5400-496 800/µL) in low-protein transudates, high-protein transudates, and exudates, respectively (P < .0001). Neutrophil counts correlated with TNCC (r
CONCLUSIONS
CONCLUSIONS
Although effusions with <50% neutrophils are transudates, most transudates and exudates have ≥50% neutrophils, limiting the diagnostic usefulness of % neutrophils for classifying effusions. Absolute neutrophil cutoffs did not notably improve effusion classification but could warrant future studies.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
384-393Informations de copyright
© 2021 American Society for Veterinary Clinical Pathology.
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