The life in a gradient: calcium, the lncRNA SPRR2C and mir542/mir196a meet in the epidermis to regulate the aging process.


Journal

Aging
ISSN: 1945-4589
Titre abrégé: Aging (Albany NY)
Pays: United States
ID NLM: 101508617

Informations de publication

Date de publication:
02 08 2021
Historique:
received: 05 08 2020
accepted: 17 07 2021
pubmed: 3 8 2021
medline: 12 1 2022
entrez: 2 8 2021
Statut: ppublish

Résumé

The turnover of the epidermis beginning with the progenitor cells in the basal layer to the fully differentiated corneocytes is tightly regulated by calcium. Calcium more than anything else promotes the differentiation of keratinocytes which implies the need for a calcium gradient with low concentrations in the stratum basale and high concentrations in the stratum granulosum. One of the hallmarks of skin aging is a collapse of this gradient that has a direct impact on the epidermal fitness. The rise of calcium in the stratum basale reduces cell proliferation, whereas the drop of calcium in the stratum granulosum leads to a changed composition of the cornified envelope. We showed that keratinocytes respond to the calcium induced block of cell division by a large increase of the expression of several miRNAs (hsa-mir542-5p, hsa-mir125a, hsa-mir135a-5p, hsa-mir196a-5p, hsa-mir491-5p and hsa-mir552-5p). The pitfall of this rescue mechanism is a dramatic change in gene expression which causes a further impairment of the epidermal barrier. This effect is attenuated by a pseudogene (SPRR2C) that gives rise to a lncRNA. SPRR2C specifically resides in the stratum granulosum/corneum thus acting as a sponge for miRNAs.

Identifiants

pubmed: 34339392
pii: 203385
doi: 10.18632/aging.203385
pmc: PMC8386546
doi:

Substances chimiques

Cornified Envelope Proline-Rich Proteins 0
MIRN196 microRNA, human 0
MIRN542 microRNA, human 0
MicroRNAs 0
RNA, Long Noncoding 0
SPRR2C protein, human 0
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

19127-19144

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Auteurs

Sven Breunig (S)

Procomcure Biotech, Breitwies, Thalgau, Austria.
Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Veronika Wallner (V)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Katharina Kobler (K)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Herbert Wimmer (H)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Peter Steinbacher (P)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Maria Karolin Streubel (MK)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Johannes Bischof (J)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.
EB House Austria, Research Program for Molecular Therapy of Genodermatoses, Department of Dermatology and Allergology, University Hospital of the Paracelsus Medical University Salzburg, Salzburg, Austria.

Jutta Duschl (J)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Claudia Neuhofer (C)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Wolfgang Gruber (W)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Fritz Aberger (F)

Department of Biosciences, Cancer Cluster Salzburg, Paris-Lodron University Salzburg, Salzburg, Austria.

Michael Breitenbach (M)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Elisabeth Russe (E)

Department of Plastic and Reconstructive Surgery, Hospital of the Barmherzige Brüder, Paracelsus Medical University Salzburg, Salzburg, Austria.

Gottfried Wechselberger (G)

Department of Plastic and Reconstructive Surgery, Hospital of the Barmherzige Brüder, Paracelsus Medical University Salzburg, Salzburg, Austria.

Albert Duranton (A)

L'Oréal Research and Innovation, Aulnay-sous-Bois, France.

Klaus Richter (K)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

Mark Rinnerthaler (M)

Department of Biosciences, Paris-Lodron University Salzburg, Salzburg, Austria.

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Classifications MeSH