Evaluation of low-cost viral concentration methods in wastewaters: Implications for SARS-CoV-2 pandemic surveillances.
Aluminum polychloride flocculation
BCoV
PP7
Polyethylene glycol precipitation
Skimmed milk flocculation
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
19
05
2021
revised:
28
07
2021
accepted:
28
07
2021
pubmed:
3
8
2021
medline:
25
11
2021
entrez:
2
8
2021
Statut:
ppublish
Résumé
In the pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) many strategies have been performed in order to control viral spread in the population and known the real-time situation about the number of infected persons. In this sense, Wastewater Based Epidemiology (WBE) has been applied as an excellent tool to evaluate the virus circulation in a population. In order to obtain reliable results, three low-cost viral concentration methods were evaluated in this study, polyethylene glycol (PEG) precipitation, skimmed milk flocculation (SM) and Aluminum polychloride flocculation, for Pseudomonas aeruginosa bacteriophage PP7 as a surrogate for non-enveloped viruses and Bovine Coronavirus (BCoV) as a surrogate for enveloped virus, with emphasis for SARS- CoV-2. Our results suggest that PEG precipitation for viral concentration, for both enveloped and non-enveloped virus from wastewater is an appropriate approach since it was more sensitive compared to SM flocculation and Aluminum polychloride flocculation. This methodology can be used for WBE studies in order to follow the epidemiology of the SARS-CoV-2 pandemic, mainly in developing countries where the economic resources are frequently limited.
Identifiants
pubmed: 34339765
pii: S0166-0934(21)00188-9
doi: 10.1016/j.jviromet.2021.114249
pmc: PMC8324412
pii:
doi:
Substances chimiques
Waste Water
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
114249Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
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