Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry.
Journal
Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
01
10
2020
accepted:
31
05
2021
pubmed:
4
8
2021
medline:
14
10
2021
entrez:
3
8
2021
Statut:
ppublish
Résumé
Of the many metabolites involved in any clinical condition, only a narrow range of biomarkers is currently being used in the clinical setting. A key to personalized medicine would be to extend this range. Metabolic fingerprinting provides a more comprehensive insight, but many methods used for metabolomics analysis are too complex and time-consuming to be diagnostically useful. Here, a rapid evaporative ionization mass spectrometry (REIMS) system for direct ex vivo real-time analysis of biofluids with minor sample pretreatment is detailed. The REIMS can be linked to various laser wavelength systems (such as optical parametric oscillator or CO
Identifiants
pubmed: 34341579
doi: 10.1038/s41596-021-00580-8
pii: 10.1038/s41596-021-00580-8
doi:
Banques de données
figshare
['10.6084/m9.figshare.14258423.v1', '10.6084/m9.figshare.14258438.v1', '10.6084/m9.figshare.14258459.v2']
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4327-4354Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature Limited.
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