A Highly Ordered, Nanostructured Fluorinated CaP-Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration.
3D printing
bones
melt electrowriting
periodontal regeneration
periodontitis
scaffolds
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
20
07
2021
received:
11
06
2021
pubmed:
4
8
2021
medline:
16
11
2021
entrez:
3
8
2021
Statut:
ppublish
Résumé
Periodontitis is a chronic inflammatory, bacteria-triggered disorder affecting nearly half of American adults. Although some level of tissue regeneration is realized, its low success in complex cases demands superior strategies to amplify regenerative capacity. Herein, highly ordered scaffolds are engineered via Melt ElectroWriting (MEW), and the effects of strand spacing, as well as the presence of a nanostructured fluorinated calcium phosphate (F/CaP) coating on the adhesion/proliferation, and osteogenic differentiation of human-derived periodontal ligament stem cells, are investigated. Upon initial cell-scaffold interaction screening aimed at defining the most suitable design, MEW poly(ε-caprolactone) scaffolds with 500 µm strand spacing are chosen. Following an alkali treatment, scaffolds are immersed in a pre-established solution to allow for coating formation. The presence of a nanostructured F/CaP coating leads to a marked upregulation of osteogenic genes and attenuated bacterial growth. In vivo findings confirm that the F/CaP-coated scaffolds are biocompatible and lead to periodontal regeneration when implanted in a rat mandibular periodontal fenestration defect model. In aggregate, it is considered that this work can contribute to the development of personalized scaffolds capable of enabling tissue-specific differentiation of progenitor cells, and thus guide simultaneous and coordinated regeneration of soft and hard periodontal tissues, while providing antimicrobial protection.
Identifiants
pubmed: 34342173
doi: 10.1002/adhm.202101152
pmc: PMC8568633
mid: NIHMS1730460
doi:
Substances chimiques
Polyesters
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2101152Subventions
Organisme : NIDCR NIH HHS
ID : K08 DE023552
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE026578
Pays : United States
Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
BMC Oral Health. 2019 Feb 1;19(1):28
pubmed: 30709394
J Dent Res. 2015 Sep;94(9 Suppl):153S-7S
pubmed: 26124215
Clin Oral Investig. 2016 Nov;20(8):1921-1933
pubmed: 26612403
Biomaterials. 2005 May;26(15):2407-13
pubmed: 15585244
Tissue Eng Part A. 2014 Apr;20(7-8):1188-96
pubmed: 24147894
Sci Adv. 2020 Mar 25;6(13):eaay7608
pubmed: 32232154
ACS Appl Mater Interfaces. 2016 Jun 22;8(24):14966-74
pubmed: 26551558
Biomaterials. 2011 Feb;32(6):1583-90
pubmed: 21112625
Acta Biomater. 2007 Sep;3(5):745-56
pubmed: 17392042
MRS Bull. 2011 May;36(5):347-355
pubmed: 22125358
Dent Mater. 2012 Jul;28(7):703-21
pubmed: 22592164
J Dent Res. 2009 Dec;88(12):1065-76
pubmed: 19887682
Acta Biomater. 2020 Sep 1;113:164-176
pubmed: 32540497
Acta Biomater. 2012 Feb;8(2):474-87
pubmed: 21856456
J Biomed Mater Res A. 2017 Feb;105(2):640-661
pubmed: 27682265
Eur Cell Mater. 2012 Jul 24;24:90-106; discussion 106
pubmed: 22828988
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:665-675
pubmed: 30948103
Acta Biomater. 2009 Jul;5(6):2025-32
pubmed: 19269907
Mater Sci Eng C Mater Biol Appl. 2015 Jan;46:1-9
pubmed: 25491953
Langmuir. 2014 Apr 29;30(16):4633-41
pubmed: 24697600
Bioact Mater. 2017 Jun 23;2(4):208-223
pubmed: 29744431
Biomaterials. 2013 Jul;34(22):5538-51
pubmed: 23623428
Colloids Surf B Biointerfaces. 2020 Jul;191:111011
pubmed: 32334136
Materials (Basel). 2017 Sep 22;10(10):
pubmed: 28937605
Biomaterials. 2006 May;27(15):2907-15
pubmed: 16448693
Ann Biomed Eng. 2017 Jan;45(1):12-22
pubmed: 27473707
ACS Biomater Sci Eng. 2019 Jul 8;5(7):3448-3461
pubmed: 33405729
ACS Biomater Sci Eng. 2018 Jan 8;4(1):1-39
pubmed: 33418675
J Dent Res. 2014 Dec;93(12):1212-21
pubmed: 25139362
Biomater Res. 2020 Jan 03;24:2
pubmed: 31911842
Nat Commun. 2015 Apr 28;6:6933
pubmed: 25917746
J Biomed Mater Res A. 2010 Nov;95(2):588-99
pubmed: 20725973
Acta Biomater. 2011 Jan;7(1):216-24
pubmed: 20801241
Chemistry. 2016 Aug 22;22(35):12347-57
pubmed: 27460160
Int Mater Rev. 2022;67(4):347-384
pubmed: 35754978
J Periodontol. 2015 May;86(5):611-22
pubmed: 25688694
Biofabrication. 2016 Jan 29;8(1):015009
pubmed: 26824799
J Biomed Mater Res B Appl Biomater. 2005 Jan 15;72(1):94-101
pubmed: 15376187
ACS Appl Mater Interfaces. 2015 Apr 15;7(14):7644-52
pubmed: 25798788
J Tissue Eng Regen Med. 2011 Jun;5(6):e126-35
pubmed: 21312338
Am J Transl Res. 2018 Dec 15;10(12):4030-4041
pubmed: 30662648
ACS Appl Mater Interfaces. 2013 Nov 27;5(22):11900-5
pubmed: 24171453
Mater Sci Eng C Mater Biol Appl. 2014 Feb 1;35:134-43
pubmed: 24411361
ACS Nano. 2019 Apr 23;13(4):3830-3838
pubmed: 30895772
Biomaterials. 2014 Jan;35(1):113-22
pubmed: 24120045
Med Prog Technol. 1988-1989;14(3-4):205-24
pubmed: 2978593
ACS Nano. 2019 May 28;13(5):5616-5622
pubmed: 31059238
J Craniomaxillofac Surg. 2019 Apr;47(4):695-703
pubmed: 30826113
Materials (Basel). 2017 Mar 24;10(4):
pubmed: 28772697
J Dent Res. 2014 Dec;93(12):1290-5
pubmed: 25139361
Dent Mater. 2015 Sep;31(9):1038-51
pubmed: 26116414
Biomater Res. 2020 Oct 01;24:17
pubmed: 33014414
Tissue Eng Part A. 2010 Sep;16(9):2977-86
pubmed: 20412028
ACS Biomater Sci Eng. 2016 Jun 13;2(6):1039-1048
pubmed: 33429512
Int J Nanomedicine. 2011;6:197-201
pubmed: 21499417
Adv Healthc Mater. 2016 Jan 7;5(1):128-36
pubmed: 25963732