Expression of miR-200c corresponds with increased reactive oxygen species and hypoxia markers after transient focal ischemia in mice.
Glia
In situ hybridization
Penumbra
Reactive oxygen species
Stroke
microRNA
Journal
Neurochemistry international
ISSN: 1872-9754
Titre abrégé: Neurochem Int
Pays: England
ID NLM: 8006959
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
19
02
2021
revised:
24
07
2021
accepted:
26
07
2021
pubmed:
4
8
2021
medline:
5
2
2022
entrez:
3
8
2021
Statut:
ppublish
Résumé
Embolic stroke results in a necrotic core of cells destined to die, but also a peri-ischemic, watershed penumbral region of potentially salvageable brain tissue. Approaches to effectively differentiate between the ischemic and peri-ischemic zones is critical for novel therapeutic discovery to improve outcomes in survivors of stroke. MicroRNAs are a class of small non-coding RNAs regulating gene translation that have region- and cell-specific expression and responses to ischemia. We have previously reported that global inhibition of cerebral microRNA-200c after experimental stroke in mice is protective, however delineating the post-stroke sub-regional and cell-type specific patterns of post-stroke miR-200c expression are necessary to minimize off-target effects and advance translational application. Here, we detail a novel protocol to visualize regional miR-200c expression after experimental stroke, complexed with visualization of regional ischemia and markers of oxidative stress in an experimental stroke model in mice. In the present study we demonstrate that the fluorescent hypoxia indicator pimonidazole hydrochloride, the reactive-oxygen-species marker 8-hydroxy-deoxyguanosine, neuronal marker MAP2 and NeuN, and the reactive astrocyte marker GFAP can be effectively complexed to determine regional differences in ischemic injury as early as 30 min post-reperfusion after experimental stroke, and can be effectively used to distinguish ischemic core from surrounding penumbral and unaffected regions for targeted therapy. This multi-dimensional post-stroke immunofluorescent imaging protocol enables a greater degree of sub-regional mechanistic investigation, with the ultimate goal of developing more effective post-stroke pharmaceutical therapy.
Identifiants
pubmed: 34343653
pii: S0197-0186(21)00192-3
doi: 10.1016/j.neuint.2021.105146
pmc: PMC8387456
mid: NIHMS1731166
pii:
doi:
Substances chimiques
MicroRNAs
0
Mirn200 microRNA, mouse
0
Reactive Oxygen Species
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105146Subventions
Organisme : NINDS NIH HHS
ID : R01 NS107445
Pays : United States
Informations de copyright
Copyright © 2021. Published by Elsevier Ltd.
Références
J Neurosci. 2008 Nov 5;28(45):11720-30
pubmed: 18987208
Brain Res. 2004 Jul 23;1015(1-2):169-74
pubmed: 15223381
Brain Res. 2015 Sep 4;1619:91-103
pubmed: 25251595
Biomed Pharmacother. 2020 May;125:110037
pubmed: 32187964
Brain Tumor Pathol. 2016 Apr;33(2):77-88
pubmed: 26968172
J Cereb Blood Flow Metab. 2014 Jan;34(1):2-18
pubmed: 24192635
J Biol Chem. 2013 Mar 8;288(10):7105-16
pubmed: 23364798
PLoS One. 2009;4(3):e4764
pubmed: 19274095
Neurochem Res. 2015 Feb;40(2):301-7
pubmed: 24993363
Mayo Clin Proc. 2005 Oct;80(10):1326-38
pubmed: 16212146
Front Cell Neurosci. 2013 Sep 23;7:160
pubmed: 24065888
Glia. 2012 Dec;60(12):1906-14
pubmed: 22907787
Front Genet. 2019 May 16;10:478
pubmed: 31156715
J Stroke Cerebrovasc Dis. 2017 Sep;26(9):1981-1987
pubmed: 28687423
Neurol Res. 2017 Jul;39(7):612-620
pubmed: 28398146
Exp Neurol. 2015 Feb;264:1-7
pubmed: 25433215
Neurobiol Dis. 2012 Jan;45(1):555-63
pubmed: 21983159
Trends Genet. 2018 Mar;34(3):165-167
pubmed: 29361313
Open Neurol J. 2012;6:51-7
pubmed: 22754596
J Neurosci Methods. 2009 Apr 30;179(1):1-8
pubmed: 19167427
BMC Genomics. 2012 Aug 01;13:357
pubmed: 22849433
Cell. 2018 Aug 9;174(4):968-981.e15
pubmed: 30078711
Antioxid Redox Signal. 2015 Jan 10;22(2):187-202
pubmed: 24359188
Stroke. 2009 Mar;40(3 Suppl):S8-12
pubmed: 19064795
Neural Regen Res. 2020 Jun;15(6):973-979
pubmed: 31823866
J Neurosci Methods. 2004 Oct 30;139(2):203-7
pubmed: 15488233
J Cereb Blood Flow Metab. 2012 Feb;32(2):213-31
pubmed: 22086195
Acta Neuropathol. 2018 Apr;135(4):529-550
pubmed: 29302779
Curr Drug Targets. 2013 Jan 1;14(1):90-101
pubmed: 23170800
Stroke. 2010 Aug;41(8):1646-51
pubmed: 20576953
J Exp Stroke Transl Med. 2011;4(1):16-23
pubmed: 21909454
Mitochondrion. 2016 Sep;30:248-54
pubmed: 27553862
Circulation. 2019 Mar 5;139(10):e56-e528
pubmed: 30700139
IUBMB Life. 2017 Nov;69(11):877-886
pubmed: 29044995
Cell Syst. 2018 Jan 24;6(1):25-36.e5
pubmed: 29289569
J Neurosci. 2007 Apr 18;27(16):4253-60
pubmed: 17442809
Am Heart J. 1981 May;101(5):593-600
pubmed: 6164281
Eur J Biochem. 1998 May 1;253(3):743-50
pubmed: 9654074
Transl Stroke Res. 2011 Dec;2(4):633-42
pubmed: 24323684
Glia. 2010 Jul;58(9):1042-9
pubmed: 20235222
J Cereb Blood Flow Metab. 1996 Jan;16(1):170-4
pubmed: 8530550
Nucleic Acids Res. 2001 May 15;29(10):2117-26
pubmed: 11353081
Can J Physiol Pharmacol. 1999 Sep;77(9):699-706
pubmed: 10566947
Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9633-8
pubmed: 24979790
Prog Neurobiol. 2016 Sep;144:103-20
pubmed: 26455456
Nature. 2016 Jul 27;535(7613):551-5
pubmed: 27466127
Stroke. 2015 Feb;46(2):551-6
pubmed: 25604249
Neuroscience. 2008 Sep 9;155(4):1204-11
pubmed: 18620031
eNeuro. 2019 Aug 29;6(4):
pubmed: 31427401
Front Neuroanat. 2017 Dec 19;11:128
pubmed: 29311856
Front Immunol. 2017 Sep 25;8:1182
pubmed: 28993774
Front Genet. 2012 Jul 02;3:120
pubmed: 22783274
Spinal Cord. 2015 Mar;53(3):182-189
pubmed: 25448187
Mol Biol Rep. 2011 Jun;38(5):3235-42
pubmed: 20182801
N Engl J Med. 2013 May 2;368(18):1685-94
pubmed: 23534542
Neurotherapeutics. 2011 Jul;8(3):349-60
pubmed: 21671123
Nat Protoc. 2010 Jun;5(6):1061-73
pubmed: 20539282
Comput Struct Biotechnol J. 2018 Oct 29;16:479-487
pubmed: 30455857