Effects of different doses of melatonin on rat adipose derived mesenchymal stem cells.
cell viability
melatonin
mesenchymal stem cell
oxidative stress
Journal
Hormone molecular biology and clinical investigation
ISSN: 1868-1891
Titre abrégé: Horm Mol Biol Clin Investig
Pays: Germany
ID NLM: 101538885
Informations de publication
Date de publication:
04 Aug 2021
04 Aug 2021
Historique:
received:
15
02
2021
accepted:
06
07
2021
pubmed:
4
8
2021
medline:
22
3
2022
entrez:
3
8
2021
Statut:
epublish
Résumé
Stem cell treatment is based on Melatonin which is crucial for lots of pathological and physiological pathways. Our aim is determining the most appropriate dose of melatonin affecting the rat adipose tissue mesenchymal stem cells. Stem cells were isolated from male rat adipose tissue. Differentiation and characterization experiments were performed. Cell viability analyses in stem cells were used the XTT [2,3-Bis-(2-methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide] assay. After 24 h incubation, different concentrations (0.5, 1, 5, 10, 50 µM) of extract were treated to the stem cells for 24 h, 48 and 72 h considering time and dose dependent manner. Total antioxidant status (TAS) and the total oxidant status (TOS) in control cells and melatonin treated cells (5, 10 µM) were determined Rel Assay commercial kits. In 24 h, melatonin increased cell viability in all groups. When we evaluate the effect of melatonin in 48 h, the most proliferation increase was seen at 5, 10 µM doses. When the total oxidant activity melatonin was found to be significantly lower in 5 and 10 µM dose groups of melatonin. Melatonin increases the survivor of stem cells and the most effective dose is 5 and 10 µM. The reduction of the oxidative stress index as a result of treating melatonin to mesenchymal stem cells showed that melatonin is a powerful antioxidant for stem cells.
Identifiants
pubmed: 34344063
pii: hmbci-2021-0013
doi: 10.1515/hmbci-2021-0013
doi:
Substances chimiques
Melatonin
JL5DK93RCL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
395-401Informations de copyright
© 2021 Walter de Gruyter GmbH, Berlin/Boston.
Références
Dominici, M, Le Blanc, K, Mueller, I, Slaper-Cortenbach, I, Marini, F, Krause, D, et al.. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy 2006;8:315–7. https://doi.org/10.1080/14653240600855905. PMID: 16923606.
Si, Z, Wang, X, Sun, C, Kang, Y, Xu, J, Wang, X, et al.. Adipose-derived stem cells: sources, potency, and implications for regenerative therapies. Biomed Pharmacother 2019;114:108765. https://doi.org/10.1016/j.biopha.2019.108765. Epub 2019 Mar 25. PMID: 30921703.
Heo, JS, Pyo, S, Lim, JY, Yoon, DW, Kim, BY, Kim, JH, et al.. Biological effects of melatonin on human adipose-derived mesenchymal stem cells. Int J Mol Med 2019;44:2234–44. https://doi.org/10.3892/ijmm.2019.4356. Epub 2019 Sep 27. PMID: 31573052; PMCID: PMC6844604.
Shuai, Y, Liao, L, Su, X, Yu, Y, Shao, B, Jing, H, et al.. Melatonin treatment improves mesenchymal stem cells therapy by preserving stemness during long-term in vitro expansion. Theranostics 2016;6:1899–917. https://doi.org/10.7150/thno.15412. PMID: 27570559; PMCID: PMC4997245.
Li, H, Zhang, Y, Liu, S, Li, F, Wang, B, Wang, J, et al.. Melatonin enhances proliferation and modulates differentiation of neural stem cells via autophagy in hyperglycemia. Stem Cell 2019;37:504–15. https://doi.org/10.1002/stem.2968. Epub 2019 Jan 14. PMID: 30644149.
Rafat, A, Roushandeh, AM, Alizadeh, A, Hashemi-Firouzi, N, Golipoor, Z. Comparison of the melatonin preconditioning efficacy between bone marrow and adipose-derived mesenchymal stem cells. Cell J 2019;20:450–8. https://doi.org/10.22074/cellj.2019.5507. Epub 2018 Aug 1. PMID: 30123990; PMCID: PMC6099139.
Tan, SS, Han, X, Sivakumaran, P, Lim, SY, Morrison, WA. Melatonin protects human adipose-derived stem cells from oxidative stress and cell death. Arch Plast Surg 2016;43:237–41. https://doi.org/10.5999/aps.2016.43.3.237. Epub 2016 May 18. PMID: 27218020; PMCID: PMC4876151.
Lee, JH, Han, YS, Lee, SH. Potentiation of biological effects of mesenchymal stem cells in ischemic conditions by melatonin via upregulation of cellular prion protein expression. J Pineal Res 2017;62:e12385. https://doi.org/10.1111/jpi.12385. Epub 2017 Jan 30. Erratum in: J Pineal Res. 2020 May;68(4):e12649. PMID: 28095625.
Fu, J, Zhao, SD, Liu, HJ, Yuan, QH, Liu, SM, Zhang, YM, et al.. Melatonin promotes proliferation and differentiation of neural stem cells subjected to hypoxia in vitro. J Pineal Res 2011;51:104–12. https://doi.org/10.1111/j.1600-079X.2011.00867.x. Epub 2011 Mar 11. PMID: 21392094.
Zhang, L, Su, P, Xu, C, Chen, C, Liang, A, Du, K, et al.. Melatonin inhibits adipogenesis and enhances osteogenesis of human mesenchymal stem cells by suppressing PPARγ expression and enhancing Runx2 expression. J Pineal Res 2010;49:364–72. https://doi.org/10.1111/j.1600-079X.2010.00803.x. Epub 2010 Aug 24. PMID: 20738756.
Rashed, LA, Elattar, S, Eltablawy, N, Ashour, H, Mahmoud, LM, El-Esawy, Y. Mesenchymal stem cells pretreated with melatonin ameliorate kidney functions in a rat model of diabetic nephropathy. Biochem Cell Biol 2018;96:564–71. https://doi.org/10.1139/bcb-2017-0230. Epub 2018 Feb 9. PMID: 29425466.
Mias, C, Trouche, E, Seguelas, MH, Calcagno, F, Dignat-George, F, Sabatier, F, et al.. Ex vivo pretreatment with melatonin improves survival, proangiogenic/mitogenic activity, and efficiency of mesenchymal stem cells injected into ischemic kidney. Stem Cell 2008;26:1749–57. https://doi.org/10.1634/stemcells.2007-1000. Epub 2008 May 8. PMID: 18467662.
Zhou, L, Chen, X, Liu, T, Gong, Y, Chen, S, Pan, G, et al.. Melatonin reverses H2O2-induced premature senescence in mesenchymal stem cells via the SIRT1-dependent pathway. J Pineal Res 2015;59:190–205. https://doi.org/10.1111/jpi.12250. Epub 2015 Jul 7. PMID: 25975679; PMCID: PMC4523475.
Winiarska, K, Dzik, JM, Labudda, M, Focht, D, Sierakowski, B, Owczarek, A, et al.. Melatonin nephroprotective action in Zucker diabetic fatty rats involves its inhibitory effect on NADPH oxidase. J Pineal Res 2016;60:109–17. https://doi.org/10.1111/jpi.12296. Epub 2015 Nov 20. PMID: 26514550.
Zhao, L, Hu, C, Zhang, P, Jiang, H, Chen, J. Melatonin preconditioning is an effective strategy for mesenchymal stem cell-based therapy for kidney disease. J Cell Mol Med 2020;24:25–33. https://doi.org/10.1111/jcmm.14769. Epub 2019 Nov 20. PMID: 31747719; PMCID: PMC6933322.
Luchetti, F, Canonico, B, Bartolini, D, Arcangeletti, M, Ciffolilli, S, Murdolo, G, et al.. Melatonin regulates mesenchymal stem cell differentiation: a review. J Pineal Res 2014;56:382–97. https://doi.org/10.1111/jpi.12133. Epub 2014 Apr 15. PMID: 24650016.
Wang, FW, Wang, Z, Zhang, YM, Du, ZX, Zhang, XL, Liu, Q, et al.. Protective effect of melatonin on bone marrow mesenchymal stem cells against hydrogen peroxide-induced apoptosis in vitro. J Cell Biochem 2013;114:2346–55. https://doi.org/10.1002/jcb.24582. PMID: 23824714.
Zhu, P, Liu, J, Shi, J, Zhou, Q, Liu, J, Zhang, X, et al.. Melatonin protects ADSCs from ROS and enhances their therapeutic potency in a rat model of myocardial infarction. J Cell Mol Med 2015;19:2232–43. https://doi.org/10.1111/jcmm.12610. Epub 2015 Jun 17. PMID: 26081690; PMCID: PMC4568927.
Lee, SJ, Jung, YH, Oh, SY, Yun, SP, Han, HJ. Melatonin enhances the human mesenchymal stem cells motility via melatonin receptor 2 coupling with Gαq in skin wound healing. J Pineal Res 2014;57:393–407. https://doi.org/10.1111/jpi.12179. Epub 2014 Oct 7. PMID: 25250716.
Chang, CL, Sung, PH, Sun, CK, Chen, CH, Chiang, HJ, Huang, TH, et al.. Protective effect of melatonin-supported adipose-derived mesenchymal stem cells against small bowel ischemia-reperfusion injury in rat. J Pineal Res. 2015;59:206–20. https://doi.org/10.1111/jpi.12251. Epub 2015 Jun 24. PMID: 26013733.
Chen, HH, Lin, KC, Wallace, CG, Chen, YT, Yang, CC, Leu, S, et al.. Additional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury. J Pineal Res. 2014;57:16–32. https://doi.org/10.1111/jpi.12140. Epub 2014 May 15. PMID: 24761983.
Yun, SP, Han, YS, Lee, JH, Kim, SM, Lee, SH. Melatonin rescues mesenchymal stem cells from senescence induced by the uremic toxin p-cresol via inhibiting mTOR-dependent autophagy. Biomol Ther (Seoul) 2018;26:389–98. https://doi.org/10.4062/biomolther.2017.071. PMID: 28655071; PMCID: PMC6029684.
Gutierrez-Cuesta, J, Tajes, M, Jiménez, A, Coto-Montes, A, Camins, A, Pallàs, M. Evaluation of potential pro-survival pathways regulated by melatonin in a murine senescence model. J Pineal Res 2008;45:497–505. https://doi.org/10.1111/j.1600-079X.2008.00626.x. Epub 2008 Aug 13. PMID: 18705649.
Liu, X, Xu, Y, Chen, S, Tan, Z, Xiong, K, Li, Y, et al.. Rescue of proinflammatory cytokine-inhibited chondrogenesis by the antiarthritic effect of melatonin in synovium mesenchymal stem cells via suppression of reactive oxygen species and matrix metalloproteinases. Free Radic Biol Med 2014;68:234–46. https://doi.org/10.1016/j.freeradbiomed.2013.12.012. Epub 2013 Dec 25. PMID: 24374373.
Hu, C, Li, L. Melatonin plays critical role in mesenchymal stem cell-based regenerative medicine in vitro and in vivo. Stem Cell Res Ther 2019;10:13. https://doi.org/10.1186/s13287-018-1114-8. PMID: 30635065; PMCID: PMC6329089.