Technological Prospecting: Mapping Patents on L-asparaginases from Extremophilic Microorganisms.

Acrylamide L-asparaginase L-asparagine amidohydrolase chemotherapeutic drug extremophiles patents technology innovation.

Journal

Recent patents on biotechnology
ISSN: 2212-4012
Titre abrégé: Recent Pat Biotechnol
Pays: United Arab Emirates
ID NLM: 101309942

Informations de publication

Date de publication:
2021
Historique:
received: 06 01 2021
revised: 30 04 2021
accepted: 01 06 2021
pubmed: 7 8 2021
medline: 28 12 2021
entrez: 6 8 2021
Statut: ppublish

Résumé

L-asparaginase (L-ASNase, L-asparagine amidohydrolase, E.C.3.5.1.1) is an enzyme with wide therapeutic applicability. Currently, the commercialized L-ASNase comes from mesophilic organisms, presenting low specificity to the substrate and limitations regarding thermostability and active pH range. Such factors prevent the maximum performance of the enzyme in different applications. Therefore, extremophilic organisms may represent important candidates for obtaining amidohydrolases with particular characteristics desired by the biotechnological market. The present study aims to carry out a technological prospecting of patents related to the L-asparaginases derived from extremophilic organisms, contributing to pave the way for further rational investigation and application of such enzymes. This patent literature review used six patents databases: The LENS, WIPO, EPO, USPTO, Patent Inspiration, and INPI. It was analyzed 2860 patents, and 14 were selected according to combinations of descriptors and study criteria. Approximately 57.14% of the patents refer to enzymes obtained from archaea, especially from the speciesPyrococcus yayanosii (35.71% of the totality). The present prospective study has singular relevance since there are no recent patent reviews for L-asparaginases, especially produced by extremophilic microorganisms. Although such enzymes have well-defined applications, corroborated by the patents compiled in this review, the most recent studies allude to new uses, such as the treatment of infections. The characterization of the catalytic profiles allows us to infer that there are potential sources still unexplored. Hence, the search for new L-ASNases with different characteristics will continue to grow in the coming years and, possibly, ramifications of the technological routes will be witnessed.

Sections du résumé

BACKGROUND BACKGROUND
L-asparaginase (L-ASNase, L-asparagine amidohydrolase, E.C.3.5.1.1) is an enzyme with wide therapeutic applicability. Currently, the commercialized L-ASNase comes from mesophilic organisms, presenting low specificity to the substrate and limitations regarding thermostability and active pH range. Such factors prevent the maximum performance of the enzyme in different applications. Therefore, extremophilic organisms may represent important candidates for obtaining amidohydrolases with particular characteristics desired by the biotechnological market.
OBJECTIVES OBJECTIVE
The present study aims to carry out a technological prospecting of patents related to the L-asparaginases derived from extremophilic organisms, contributing to pave the way for further rational investigation and application of such enzymes.
METHODS METHODS
This patent literature review used six patents databases: The LENS, WIPO, EPO, USPTO, Patent Inspiration, and INPI.
RESULTS RESULTS
It was analyzed 2860 patents, and 14 were selected according to combinations of descriptors and study criteria. Approximately 57.14% of the patents refer to enzymes obtained from archaea, especially from the speciesPyrococcus yayanosii (35.71% of the totality).
CONCLUSION CONCLUSIONS
The present prospective study has singular relevance since there are no recent patent reviews for L-asparaginases, especially produced by extremophilic microorganisms. Although such enzymes have well-defined applications, corroborated by the patents compiled in this review, the most recent studies allude to new uses, such as the treatment of infections. The characterization of the catalytic profiles allows us to infer that there are potential sources still unexplored. Hence, the search for new L-ASNases with different characteristics will continue to grow in the coming years and, possibly, ramifications of the technological routes will be witnessed.

Identifiants

pubmed: 34353277
pii: BIOT-EPUB-117107
doi: 10.2174/1872208315666210805162459
doi:

Substances chimiques

Asparagine 7006-34-0
Asparaginase EC 3.5.1.1

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

250-265

Subventions

Organisme : national country for scientific reacher CNPq
ID : 433388/2018-8

Informations de copyright

Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

Auteurs

Igor Gomes de Oliveira Lima (IG)

Laboratorio de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil.

James Romero Soares Bispo (JRS)

Laboratorio de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil.

Maurício Bernardo da Silva (MB)

Laboratorio de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil.

Alexya de Oliveira Feitosa (A)

Laboratorio de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil.

Ana Caroline Melo Dos Santos (ACM)

Laboratorio de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil.

Magna Suzana Alexandre Moreira (MSA)

Laboratorio de Farmacologia e Imunologia, Instituto de Ciencias Biologicas e da Saude, Universidade Federal de Alagoas, 57072-900, Maceio, AL, Brazil.

Michel Rodrigo Zambrano Passarini (MRZ)

Universidade Federal da Integracao Latino-Americana, 85870-650, Foz Do Iguacu, PR, Brazil.

Paulo Eduardo Aguiar Saraiva Câmara (PEA)

Departamento de Botanica, Campus Darcy Ribeiro, Universidade de Brasília, Asa Norte, s/n, 70910-900, Brasilia, DF, Brazil.

Luiz Henrique Rosa (LH)

Departamento de Microbiologia, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil.

Valéria Maia Oliveira (VM)

Divisao de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas. Universidade Estadual de Campinas, UNICAMP, 13148-218, Paulinia, SP, Brazil.

Aline Cavalcanti de Queiroz (AC)

Laboratorio de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil.

Alysson Wagner Fernandes Duarte (AWF)

Laboratorio de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil.

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Classifications MeSH