Yippee like 4 (Ypel4) is essential for normal mouse red blood cell membrane integrity.
Anemia
/ metabolism
Animals
Anion Exchange Protein 1, Erythrocyte
/ metabolism
Carrier Proteins
/ genetics
Erythrocyte Membrane
/ genetics
Erythrocytes
/ metabolism
Erythrocytes, Abnormal
/ metabolism
Erythropoiesis
/ genetics
Female
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Polycythemia
/ genetics
Spleen
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 08 2021
05 08 2021
Historique:
received:
07
05
2021
accepted:
22
07
2021
entrez:
6
8
2021
pubmed:
7
8
2021
medline:
9
11
2021
Statut:
epublish
Résumé
The YPEL family genes are highly conserved across a diverse range of eukaryotic organisms and thus potentially involved in essential cellular processes. Ypel4, one of five YPEL family gene orthologs in mouse and human, is highly and specifically expressed in late terminal erythroid differentiation (TED). In this study, we investigated the role of Ypel4 in murine erythropoiesis, providing for the first time an in-depth description of a Ypel4-null phenotype in vivo. We demonstrated that the Ypel4-null mice displayed a secondary polycythemia with macro- and reticulocytosis. While lack of Ypel4 did not affect steady-state TED in the bone marrow or spleen, the anemia-recovering capacity of Ypel4-null cells was diminished. Furthermore, Ypel4-null red blood cells (RBC) were cleared from the circulation at an increased rate, demonstrating an intrinsic defect of RBCs. Scanning electron micrographs revealed an ovalocytic morphology of Ypel4-null RBCs and functional testing confirmed reduced deformability. Even though Band 3 protein levels were shown to be reduced in Ypel4-null RBC membranes, we could not find support for a physical interaction between YPEL4 and the Band 3 protein. In conclusion, our findings provide crucial insights into the role of Ypel4 in preserving normal red cell membrane integrity.
Identifiants
pubmed: 34354145
doi: 10.1038/s41598-021-95291-1
pii: 10.1038/s41598-021-95291-1
pmc: PMC8342551
doi:
Substances chimiques
Anion Exchange Protein 1, Erythrocyte
0
Carrier Proteins
0
YPEL4 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15898Informations de copyright
© 2021. The Author(s).
Références
Nat Biotechnol. 2020 Mar;38(3):276-278
pubmed: 32055031
Am Fam Physician. 2018 Sep 15;98(6):354-361
pubmed: 30215915
Proc Natl Acad Sci U S A. 2008 Jun 10;105(23):8026-31
pubmed: 18524950
Biochem Cell Biol. 2010 Jun;88(3):445-50
pubmed: 20555386
Nature. 2016 Sep 22;537(7621):508-514
pubmed: 27626380
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Adv Bioinformatics. 2008;2008:420747
pubmed: 19956698
Blood. 2001 Sep 1;98(5):1577-84
pubmed: 11520810
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1542-6
pubmed: 4104431
Blood Rev. 1989 Mar;3(1):59-65
pubmed: 2650777
Blood Cells Mol Dis. 2010 Jun 15;45(1):1-8
pubmed: 20346715
Mol Cell Neurosci. 2009 Jan;40(1):111-9
pubmed: 19013529
Cells. 2020 Jun 26;9(6):
pubmed: 32604778
Cytometry B Clin Cytom. 2008 Jul;74(4):244-50
pubmed: 18454487
Insect Mol Biol. 2001 Feb;10(1):77-86
pubmed: 11240639
Genes Dev. 2012 Sep 15;26(18):2075-87
pubmed: 22929040
Int J Lab Hematol. 2017 May;39 Suppl 1:47-52
pubmed: 28447420
Front Physiol. 2018 Jun 01;9:656
pubmed: 29910743
Exp Hematol. 1989 Mar;17(3):246-51
pubmed: 2917622
Nucleic Acids Res. 2002 Jan 1;30(1):207-10
pubmed: 11752295
Blood. 2011 Mar 24;117(12):3435-44
pubmed: 21177435
Biol Cell. 2010 Oct;102(10):561-80
pubmed: 20812916
Br J Haematol. 2000 Dec;111(3):924-33
pubmed: 11122157
Genome Biol. 2014;15(12):550
pubmed: 25516281
PLoS Biol. 2010 Sep 21;8(9):
pubmed: 20877475
Cancer Res. 2010 May 1;70(9):3566-75
pubmed: 20388804
Blood. 1977 Jun;49(6):855-64
pubmed: 861374
Cell Rep. 2016 Aug 2;16(5):1470-1484
pubmed: 27452463
Blood. 2011 Dec 8;118(24):6258-68
pubmed: 21998215
Blood. 2014 May 29;123(22):3466-77
pubmed: 24637361
Genes Cells. 2001 Jul;6(7):619-29
pubmed: 11473580
Blood. 2013 Feb 7;121(6):e5-e13
pubmed: 23243273
PLoS Genet. 2020 Jun 16;16(6):e1008841
pubmed: 32544203
Ann Hematol. 2015 Aug;94(8):1277-83
pubmed: 25907377
J Microbiol Biotechnol. 2017 Mar 28;27(3):633-643
pubmed: 28173693
Blood. 1978 Mar;51(3):527-37
pubmed: 623913
Nat Biotechnol. 2017 Apr 11;35(4):316-319
pubmed: 28398311
J Exp Clin Cancer Res. 2016 Jul 11;35(1):109
pubmed: 27400785
Nat Biotechnol. 2011 Jan;29(1):24-6
pubmed: 21221095
Blood. 2013 Feb 21;121(8):e43-9
pubmed: 23287863
Cold Spring Harb Perspect Med. 2013 Apr 01;3(4):a011601
pubmed: 23545573
Gene. 2004 Sep 29;340(1):31-43
pubmed: 15556292
Haematologica. 2020 Jan 09;105(11):2561-2571
pubmed: 33131245
Acta Haematol. 2006;116(3):186-91
pubmed: 17016037
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Int J Hematol. 2011 Feb;93(2):139-143
pubmed: 21293952
Cancer Res. 2003 Feb 1;63(3):705-12
pubmed: 12566317
Cytometry. 2002 Dec 15;50(6):313-25
pubmed: 12497593
Genomics. 2010 Aug;96(2):102-11
pubmed: 20580816