Thermostable Mutants of Glycoside Hydrolase Family 6 Cellobiohydrolase from the Basidiomycete
cellobiohydrolase
glycoside hydrolase
protein engineering
random mutagenesis
Journal
Journal of applied glycoscience
ISSN: 1880-7291
Titre abrégé: J Appl Glycosci (1999)
Pays: Japan
ID NLM: 101167786
Informations de publication
Date de publication:
2020
2020
Historique:
received:
05
04
2020
accepted:
09
06
2020
entrez:
6
8
2021
pubmed:
7
8
2021
medline:
7
8
2021
Statut:
epublish
Résumé
Thermal inactivation of saccharifying enzymes is a crucial issue for the efficient utilization of cellulosic biomass as a renewable resource. Cellobiohydrolases (CBHs) are a kind of cellulase. In general, CBHs belonging to glycoside hydrolase (GH) family 6 (Cel6) act synergistically with CBHs of GH family 7 (Cel7) and other carbohydrate-active enzymes during the degradation of cellulosic biomass. However, while the catalytic rate of enzymes generally becomes faster at higher temperatures, Cel6 CBHs are inactivated at lower temperatures than Cel7 CBHs, and this represents a limiting factor for industrial utilization. In this study, we produced a series of mutants of the glycoside hydrolase family 6 cellobiohydrolase
Identifiants
pubmed: 34354533
doi: 10.5458/jag.jag.JAG-2020_0004
pmc: PMC8132074
doi:
Types de publication
Journal Article
Langues
eng
Pagination
79-86Informations de copyright
2020 by The Japanese Society of Applied Glycoscience.
Références
Biotechnol Bioeng. 2013 Jul;110(7):1874-83
pubmed: 23404363
Biotechnol Bioeng. 2008 Oct 15;101(3):515-28
pubmed: 18512263
Biochim Biophys Acta. 2013 Aug;1834(8):1539-44
pubmed: 23676789
Nat Protoc. 2015 Jun;10(6):845-58
pubmed: 25950237
Protein Eng Des Sel. 2013 Jan;26(1):73-9
pubmed: 23091162
FEBS Lett. 1998 Jun 16;429(3):341-6
pubmed: 9662445
Appl Microbiol Biotechnol. 2012 Sep;95(6):1469-78
pubmed: 22215071
Biotechnol Biofuels. 2016 Sep 19;9:199
pubmed: 27660653
Proc Natl Acad Sci U S A. 2019 Nov 12;116(46):23061-23067
pubmed: 31666327
Science. 2011 Sep 2;333(6047):1279-82
pubmed: 21885779
Biotechnol Appl Biochem. 1998 Aug;28(1):39-45
pubmed: 9693087
Acta Crystallogr F Struct Biol Commun. 2017 Jul 01;73(Pt 7):398-403
pubmed: 28695848
Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5
pubmed: 24270786
J Biol Chem. 2011 Nov 25;286(47):41028-35
pubmed: 21965672
J Am Chem Soc. 2014 Mar 26;136(12):4584-92
pubmed: 24571226
J Biol Chem. 2009 Sep 25;284(39):26229-33
pubmed: 19625252
Yeast. 2005 Mar;22(4):249-70
pubmed: 15704221
Biochim Biophys Acta. 2014 Feb;1840(2):847-75
pubmed: 23748139
EMBO J. 2013 May 15;32(10):1478-88
pubmed: 23584533
Biotechnol Adv. 2006 Sep-Oct;24(5):452-81
pubmed: 16690241