Large Intronic Deletion of the Fragile Site Gene

PRKN common chromosomal fragile sites genome editing genomic instability large genes parkin

Journal

Frontiers in genetics
ISSN: 1664-8021
Titre abrégé: Front Genet
Pays: Switzerland
ID NLM: 101560621

Informations de publication

Date de publication:
2021
Historique:
received: 14 04 2021
accepted: 28 06 2021
entrez: 6 8 2021
pubmed: 7 8 2021
medline: 7 8 2021
Statut: epublish

Résumé

Common chromosomal fragile sites (CFSs) are genomic regions prone to form breaks and gaps on metaphase chromosomes during conditions of replication stress. Moreover, CFSs are hotspots for deletions and amplifications in cancer genomes. Fragility at CFSs is caused by transcription of extremely large genes, which contributes to replication problems. These extremely large genes do not encode large proteins, but the extreme sizes of the genes originate from vast introns. Intriguingly, the intron sizes of extremely large genes are conserved between mammals and birds. Here, we have used reverse genetics to address the function and significance of the largest intron in the extremely large gene

Identifiants

pubmed: 34354738
doi: 10.3389/fgene.2021.695172
pmc: PMC8329550
doi:

Types de publication

Journal Article

Langues

eng

Pagination

695172

Informations de copyright

Copyright © 2021 Munk, Voutsinos and Oestergaard.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer VB declared a shared affiliation with one of the authors SM to the handling editor at the time of review.

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Auteurs

Sebastian H N Munk (SHN)

Department of Biology, University of Copenhagen, Copenhagen, Denmark.

Vasileios Voutsinos (V)

Department of Biology, University of Copenhagen, Copenhagen, Denmark.

Vibe H Oestergaard (VH)

Department of Biology, University of Copenhagen, Copenhagen, Denmark.

Classifications MeSH