Identification of amyloid beta in small extracellular vesicles
Journal
Nanoscale advances
ISSN: 2516-0230
Titre abrégé: Nanoscale Adv
Pays: England
ID NLM: 101738708
Informations de publication
Date de publication:
13 Jul 2021
13 Jul 2021
Historique:
received:
05
05
2021
accepted:
07
06
2021
entrez:
6
8
2021
pubmed:
7
8
2021
medline:
7
8
2021
Statut:
epublish
Résumé
One of the hallmarks of Alzheimer's disease (AD) pathogenesis is believed to be the production and deposition of amyloid-beta (Aβ) peptide into extracellular plaques. Existing research indicates that extracellular vesicles (EVs) can carry Aβ associated with AD. However, characterization of the EVs-associated Aβ and its conformational variants has yet to be realized. Raman spectroscopy is a label-free and non-destructive method that is able to assess the biochemical composition of EVs. This study reports for the first time the Raman spectroscopic fingerprint of the Aβ present in the molecular cargo of small extracellular vesicles (sEVs). Raman spectra were measured from sEVs isolated from Alzheimer's disease cell culture model, where secretion of Aβ is regulated by tetracycline promoter, and from midbrain organoids. The averaged spectra of each sEV group showed considerable variation as a reflection of the biochemical content of sEVs. Spectral analysis identified more intense Raman peaks at 1650 cm
Identifiants
pubmed: 34355118
doi: 10.1039/d1na00330e
pii: d1na00330e
pmc: PMC8276787
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4119-4132Subventions
Organisme : NCI NIH HHS
ID : R01 CA241666
Pays : United States
Informations de copyright
This journal is © The Royal Society of Chemistry.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Nat Rev Immunol. 2002 Aug;2(8):569-79
pubmed: 12154376
Biochemistry. 1998 Aug 4;37(31):11064-77
pubmed: 9693002
Biochim Biophys Acta. 1992 Apr 8;1120(2):123-43
pubmed: 1373323
J Mol Biol. 2006 Jan 6;355(1):63-71
pubmed: 16303137
Biochim Biophys Acta. 1973 Nov 29;326(2):245-55
pubmed: 4765102
Ann Pediatr Endocrinol Metab. 2016 Sep;21(3):119-125
pubmed: 27777903
Int J Mol Sci. 2019 Jan 20;20(2):
pubmed: 30669512
NPJ Aging Mech Dis. 2016 Jun 23;2:16012
pubmed: 28721267
Biochim Biophys Acta. 2003 Mar 10;1610(2):281-90
pubmed: 12648781
Biochemistry. 2009 Mar 10;48(9):1870-7
pubmed: 19216516
J Extracell Vesicles. 2019 Apr 01;8(1):1596016
pubmed: 30988894
Nanoscale. 2015;7(20):9290-7
pubmed: 25939587
Nucleic Acids Res. 2012 Nov;40(21):10937-49
pubmed: 22965126
Acta Neuropathol. 2018 Jul;136(1):41-56
pubmed: 29934873
Nanomedicine. 2020 Oct;29:102249
pubmed: 32599162
Acta Neuropathol Commun. 2020 Dec 11;8(1):222
pubmed: 33308303
Biosens Bioelectron. 2019 Oct 1;142:111503
pubmed: 31376716
Phys Biol. 2009 Feb 10;6(1):015005
pubmed: 19208933
Neurobiol Aging. 2020 Feb;86:102-111
pubmed: 31883770
Sci Transl Med. 2013 Sep 4;5(201):201ra119
pubmed: 24005159
Nanotechnology. 2018 Apr 3;29(13):132001
pubmed: 29376505
Cells. 2019 Feb 12;8(2):
pubmed: 30759880
Front Aging Neurosci. 2017 Feb 01;9:12
pubmed: 28203202
Neurobiol Dis. 2019 Jan;121:1-16
pubmed: 30218757
Brain Pathol. 2001 Jan;11(1):1-11
pubmed: 11145195
J Extracell Vesicles. 2015 Dec 07;4:28533
pubmed: 26649679
J Biol Chem. 2009 Dec 4;284(49):34211-22
pubmed: 19801663
J Am Chem Soc. 2004 Mar 3;126(8):2399-408
pubmed: 14982446
Anal Chem. 2018 Oct 2;90(19):11290-11296
pubmed: 30157378
PLoS One. 2020 Jul 2;15(7):e0235214
pubmed: 32614841
ACS Chem Neurosci. 2018 Mar 21;9(3):404-420
pubmed: 29308873
Sci Rep. 2015 Aug 27;5:13489
pubmed: 26311128
R Soc Open Sci. 2018 Dec 12;5(12):181483
pubmed: 30662753
Protein Cell. 2019 Apr;10(4):295-299
pubmed: 29616487
Biochemistry. 2014 Oct 21;53(41):6550-65
pubmed: 25284680
Analyst. 2020 Jan 20;145(2):626-635
pubmed: 31782420
Dis Model Mech. 2011 Sep;4(5):634-48
pubmed: 21596710
Chem Sci. 2019 Nov 14;11(2):525-533
pubmed: 32190272
Nanomedicine. 2019 Apr;17:137-149
pubmed: 30703535
Int J Pharm. 2009 Oct 1;380(1-2):206-15
pubmed: 19589376
Biochem J. 2004 May 15;380(Pt 1):161-71
pubmed: 14965343
Oncotarget. 2017 Jul 28;8(49):85290-85310
pubmed: 29156720
Sci Rep. 2017 Aug 29;7(1):9820
pubmed: 28852131
Biochem J. 2009 Jul 15;421(3):415-23
pubmed: 19435461
J Extracell Vesicles. 2015 Nov 11;4:29260
pubmed: 26563735
J Extracell Vesicles. 2019 Jan 27;8(1):1568780
pubmed: 30728924
Front Chem. 2019 May 07;7:279
pubmed: 31134179
Biochim Biophys Acta. 1976 Sep 7;443(3):331-8
pubmed: 183820
J Clin Invest. 2016 Apr 1;126(4):1208-15
pubmed: 27035812
J Mater Chem B. 2020 Oct 7;8(38):8845-8852
pubmed: 33026405
JAMA Neurol. 2019 Nov 01;76(11):1340-1351
pubmed: 31305918
Nanomedicine. 2019 Nov;22:102097
pubmed: 31648040
Biochim Biophys Acta. 1977 Feb 23;486(2):217-27
pubmed: 836854
Sci Rep. 2017 May 30;7(1):2477
pubmed: 28559572
Sci Adv. 2018 Nov 16;4(11):eaat7715
pubmed: 30456301
Biochemistry. 2013 Aug 6;52(31):5176-83
pubmed: 23837615
Eur Biophys J. 2008 Mar;37(3):323-32
pubmed: 18064447
Brain Res Mol Brain Res. 1994 Oct;26(1-2):207-17
pubmed: 7854049
Nanomedicine. 2017 Apr;13(3):835-841
pubmed: 27965168
J Extracell Vesicles. 2018 Nov 23;7(1):1535750
pubmed: 30637094
Nat Protoc. 2019 Feb;14(2):576-593
pubmed: 30651586
Nat Commun. 2019 Mar 8;10(1):1144
pubmed: 30850633
ACS Chem Neurosci. 2018 Nov 21;9(11):2786-2794
pubmed: 29865787
Analyst. 2015 Aug 7;140(15):4967-80
pubmed: 26042229
J Extracell Vesicles. 2012 Nov 21;1:
pubmed: 24009887
Autoimmunity. 2016 Sep;49(6):357-365
pubmed: 27259064
J Physiol Paris. 1998 Jun-Aug;92(3-4):289-92
pubmed: 9789825
Front Physiol. 2012 May 03;3:124
pubmed: 22563321
J Neurosci. 2013 Jun 19;33(25):10512-25
pubmed: 23785163
J Extracell Vesicles. 2020 Jan 7;9(1):1710020
pubmed: 32002172
Sci Rep. 2019 May 8;9(1):7089
pubmed: 31068645
J Neurosci. 2019 Nov 20;39(47):9269-9273
pubmed: 31748282
ACS Sens. 2020 Sep 25;5(9):2820-2833
pubmed: 32935542
Alzheimers Dement. 2015 Jun;11(6):600-7.e1
pubmed: 25130657
Proc Natl Acad Sci U S A. 2012 May 15;109(20):7717-22
pubmed: 22547798
Analyst. 2009 Jun;134(6):1232-9
pubmed: 19475153
Circ Res. 2017 May 12;120(10):1649-1657
pubmed: 28495995
Biopolymers. 2003;72(4):230-40
pubmed: 12833477
Sci Rep. 2017 Jul 21;7(1):6186
pubmed: 28733621
J Biophotonics. 2015 Jul;8(7):584-96
pubmed: 25256347
Annu Rev Neurosci. 2011;34:185-204
pubmed: 21456963
Lab Chip. 2019 Jul 23;19(15):2526-2536
pubmed: 31292600
J Extracell Vesicles. 2016 Oct 31;5:32945
pubmed: 27802845
Philos Trans R Soc Lond B Biol Sci. 2014 Sep 26;369(1652):
pubmed: 25135977
Sci Rep. 2013 Dec 02;3:3396
pubmed: 24292236
Nat Protoc. 2020 Jan;15(1):102-121
pubmed: 31836866
Lab Chip. 2019 Oct 7;19(19):3326-3336
pubmed: 31497821
Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11172-7
pubmed: 16837572
Front Neurosci. 2019 Nov 26;13:1208
pubmed: 31849573